English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/35220
Title: Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace
Authors: Silvar, Cristina; Dhif, Hichem; Igartua Arregui, Ernesto; Kopahnke, Doris; Gracia Gimeno, María Pilar; Lasa Dolhagaray, José Manuel; Ordon, Frank; Casas Cendoya, Ana María
Keywords: Barley
Powdery mildew
Disease resistance
Quantitative trait locus
Issue Date: Apr-2010
Publisher: Springer
Citation: Silvar C, Dhif H, Igartua E, Kopah D, Gracia MP, Lasa JM, Ordon F, Casas AM. Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace. Molecular breeding 25 (4): 581-592 (2010)
Abstract: The Spanish landrace-derived inbred line SBCC97, together with other lines from the Spanish Barley Core Collection, displays high resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a recombinant inbred line population derived from a cross between SBCC97 and the susceptible cultivar ‘Plaisant’. Phenotypic analysis was performed using four B. graminis isolates, and genetic maps were constructed with mainly simple sequence repeat (SSR) markers, following a sequential genotyping strategy. Two major QTLs with large effects were identified on chromosome 7H, and they accounted for up to 45% of the total phenotypic variance. The alleles for resistance at each QTL were contributed by the Spanish parent SBCC97. One locus was mapped to the short arm of chromosome 7HS, and was flanked by the resistance gene analogue (RGA) marker S9202 and the SSR GBM1060. This corresponded to the same chromosomal region in which a major race-specific resistance gene from Hordeum vulgare ssp. spontaneum, designated as mlt, had been identified previously. The second QTL was linked tightly to marker EBmac0755, and it shared its chromosomal location with the qualitative resistance gene Mlf, which has only been described previously in the wild ancestor H. spontaneum. This is the first report of these two QTLs occurring together in cultivated barley, and it paves the way for their use in barley breeding programs that are designed to transfer resistance alleles into elite cultivars.
Description: 30 Pag., 1 Tabl., 5 Fig. The definitive version is available at: http://www.springerlink.com/content/1380-3743/
Publisher version (URL): http://dx.doi.org/10.1007/s11032-009-9354-z
URI: http://hdl.handle.net/10261/35220
ISSN: 1380-3743
DOI: 10.1007/s11032-009-9354-z
E-ISSN: 1572-9788
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
SilvarC_MolBreed_2010.pdf254,21 kBAdobe PDFThumbnail
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.