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Closed Access item Binding of polylysine to protein kinase CK2, measured by Surface Plasmon Resonance
|Authors:||Benítez, María J.|
Briones Fernández-Pola, Fernando
Moreno, Francisco J.
Jiménez, Juan S.
|Keywords:||Polylysine, CK2, Surface Plasmon Research|
|Citation:||Molecular and Cellular Biochemistry 191 (1): 29-33 (1999)|
|Abstract:||The interaction between protein kinase CK2 and polylysine has been studied by Surface Plasmon Resonance (SPR). The binding process has a very low energy of activation, it is irreversible, and too slow as to explain the enzyme activity stimulation as a direct consequence of the polylysine binding. The polylysine interaction with a peptide substrate and with casein are faster, and in agreement with a substrate-mediated mechanism of activity stimulation. After several hours of incubation, the binding of polylysine to CK2 produces the loss of enzymatic activity.|
|Publisher version (URL):||http://dx.doi.org/10.1023/A:1006821520346|
|E-ISSNmetadata.dc.identifier.doi = DOI:||1573-4919|
|Appears in Collections:||(IMM-CNM) Artículos|
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