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Título : Binding of polylysine to protein kinase CK2, measured by Surface Plasmon Resonance
Autor : Benítez, María J., Mier, Gerardo, Briones Fernández-Pola, Fernando, Moreno, Francisco J., Jiménez, Juan S.
Palabras clave : Polylysine
Surface Plasmon Research
Fecha de publicación : 1999
Editor: Springer
Resumen: The interaction between protein kinase CK2 and polylysine has been studied by Surface Plasmon Resonance (SPR). The binding process has a very low energy of activation, it is irreversible, and too slow as to explain the enzyme activity stimulation as a direct consequence of the polylysine binding. The polylysine interaction with a peptide substrate and with casein are faster, and in agreement with a substrate-mediated mechanism of activity stimulation. After several hours of incubation, the binding of polylysine to CK2 produces the loss of enzymatic activity.
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ISSN: 0300-8177
DOI: 10.1023/A:1006821520346
Citación : Molecular and Cellular Biochemistry 191 (1): 29-33 (1999)
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