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Título : Inositol phosphate profiling of fermented cowpeas by H-1 NMR spectroscopy
Autor : Valverde, Serafín, Frías, Juana, Doblado, Rosa, Jimeno, M. Luisa, Vidal-Valverde, Concepción
Palabras clave : Fermentation
cowpeas
inositol phosphates
1H NMR spectroscopy
Fecha de publicación : 2005
Editor: American Chemical Society
Citación : Journal of Agriciltural and Food Chemistry 53 : 4714-4721 (2005)
Resumen: The inositol phosphate content of naturally fermented cowpeas (Vigna sinensis var. Carilla) was studied using ion-pair HPLC and 1H NMR spectroscopy. The fermented flour was extracted with 0.5 M HCl, and the extract was purified and fractionated by ion-exchange chromatography. 1H NMR allowed for the identification of two monophosphates [Ins(1 or 3)P1 and Ins(4 or 6)P1], one inositol diphosphate [Ins(1,4)P2], three inositol triphosphates [Ins(1,2,6)P3, Ins(1,5,6)P3, and Ins(1,4,5)P3], one inositol tetraphosphate [Ins(1,3,4,5)P4], and one inositol pentaphosphate [Ins(1,2,3,5,6)P5]. Some of these isomers [Ins(1,4,5)P3 and Ins(1,3,4,5)P4] are considered to play important biological roles in intracellular signaling.
Versión del editor: http://dx.doi.org/10.1021/jf0481047
URI : http://hdl.handle.net/10261/34872
ISSN: 0021-8561
DOI: 10.1021/jf0481047
Citación : Journal of Agriciltural and Food Chemistry 53 : 4714-4721 (2005)
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