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Title

In vivo brain dialysis study of the somatodendritic release of serotonin in the raphe nuclei of the rat. Effects of 8-OH-DPAT

AuthorsAdell, Albert CSIC ORCID ; Carceller, Alicia; Artigas, Francesc CSIC ORCID
Keywords8-Hydroxy-2-(di-n-propylamino)tetralin
5-hydroxytryptamine
5-HT1A receptors
p-Chloroamphetamine
Raphe nuclei
Somatodendritic release
Issue DateMay-1993
PublisherJohn Wiley & Sons
CitationJournal of Neurochemistry 60 (5) : 1673-1681 (1993)
AbstractThe characteristics of the serotonin (5-HT) output in the dorsal and median raphe nuclei of the rat were studies using in vivo microdialysis. The basal output of 5-HT increased after KCl was added to the perfusion fluid. In contrast, neither the omission of calcium ions nor the addition of 0.5 microM tetrodotoxin affected dialysate 5-HT or 5-hydroxyindoleacetic acid (5-HIAA). Reserpine did not decrease the output of 5-HT and 5-HIAA 24 h later and p-chloroamphetamine increased 5-HT in both vehicle- and reserpine-treated rats severalfold. 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), at 1 or 10 microM, perfused into the raphe did not change the outputs of 5-HT or 5-HIAA. Higher doses (0.1, 1, and 10 mM) increased extracellular 5-HT in the raphe, probably via an inhibition of uptake. In animals bearing two probes (raphe nuclei and ventral hippocampus), only the 10 mM dose of 8-OH-DPAT perfused into the raphe decreased the hippocampal output of 5-HT and 5-HIAA. The systemic injection of 0.1 mg/kg 8-OH-DPAT decreased dialysate 5-HT and 5-HIAA in the raphe and hippocampus. These results suggest that extracellular 5-HT in raphe nuclei originates from a cytoplasmic pool and is not dependent on either nerve impulse of 5-HT neurons or local activation of 5-HT1A receptors.
Publisher version (URL)http://dx.doi.org/10.1111/j.1471-4159.1993.tb13390.x
URIhttp://hdl.handle.net/10261/34789
DOI10.1111/j.1471-4159.1993.tb13390.x
ISSN0022-3042
E-ISSN1471-4159
Appears in Collections:(IIBB) Artículos




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