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Closed Access item In vivo brain dialysis study of the somatodendritic release of serotonin in the raphe nuclei of the rat. Effects of 8-OH-DPAT

Authors:Adell, Albert
Carceller, Alicia
Artigas, Francesc
Keywords:8-Hydroxy-2-(di-n-propylamino)tetralin, 5-Hydroxytryptamine, 5-HT1A receptors, p-Chloroamphetamine, Raphe nuclei, Somatodendritic release
Issue Date:May-1993
Publisher:John Wiley & Sons
Citation:Journal of Neurochemistry 60 (5) : 1673-1681 (1993)
Abstract:The characteristics of the serotonin (5-HT) output in the dorsal and median raphe nuclei of the rat were studies using in vivo microdialysis. The basal output of 5-HT increased after KCl was added to the perfusion fluid. In contrast, neither the omission of calcium ions nor the addition of 0.5 microM tetrodotoxin affected dialysate 5-HT or 5-hydroxyindoleacetic acid (5-HIAA). Reserpine did not decrease the output of 5-HT and 5-HIAA 24 h later and p-chloroamphetamine increased 5-HT in both vehicle- and reserpine-treated rats severalfold. 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), at 1 or 10 microM, perfused into the raphe did not change the outputs of 5-HT or 5-HIAA. Higher doses (0.1, 1, and 10 mM) increased extracellular 5-HT in the raphe, probably via an inhibition of uptake. In animals bearing two probes (raphe nuclei and ventral hippocampus), only the 10 mM dose of 8-OH-DPAT perfused into the raphe decreased the hippocampal output of 5-HT and 5-HIAA. The systemic injection of 0.1 mg/kg 8-OH-DPAT decreased dialysate 5-HT and 5-HIAA in the raphe and hippocampus. These results suggest that extracellular 5-HT in raphe nuclei originates from a cytoplasmic pool and is not dependent on either nerve impulse of 5-HT neurons or local activation of 5-HT1A receptors.
Publisher version (URL):http://dx.doi.org/10.1111/j.1471-4159.1993.tb13390.x
URI:http://hdl.handle.net/10261/34789
ISSN:0022-3042
E-ISSNmetadata.dc.identifier.doi = DOI:1471-4159
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Appears in Collections:(IIBB) Artículos

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