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Open Access item 3-Hydroxybutyrate co-infused with noradrenaline decreases resulting plasma levels of noradrenaline in Wistar rats

Authors:Cañas, Xavier
Sanchís, Daniel
Gómez, Gloria
Casanovas, Josep M.
Artigas, Francesc
Fernández-López, José Antonio
Remesar, Xavier
Alemany, Marià
Keywords:3-hydroxybutyrate, Ketone bodies, Thermogenesis, Noradrenaline, Brown adipose tissue, Body temperature, Rat
Issue Date:Oct-1997
Publisher:Company of Biologists
Citation:Journal of Experimental Biology 200(20): 641-2646 (1997)
Abstract:Pentobarbital-anaesthetized male Wistar rats were infused with 6microgkg-1min-1 of noradrenaline. The infusion was supplemented with 8.5 mgkg-1min-1 of D-3-hydroxybutyrate (3-OHB) for 15 min in order to determine its effect on the adrenergic response of the rat. Plasma levels of noradrenaline rose to a plateau of approximately 50 nmoll-1 with infusion. In the group infused with noradrenaline alone, noradrenaline levels were maintained for 1h. Supplementation with 3-OHB induced a decrease in plasma noradrenaline level that was inversely correlated with 3-OHB level. Aortic and interscapular brown adipose tissue temperatures increased with noradrenaline infusion, but the rise was arrested by 3-OHB; replacing 3-OHB with glucose had no effect. Infusion of saline, glucose or 3-OHB in the absence of noradrenaline did not induce a rise in temperature in either tissue. Blood 3-OHB concentration increased to 1.2 mmoll-1 during 3-OHB infusion, decreasing rapidly at the end of infusion. Blood glucose levels increased with noradrenaline infusion; the presence of high 3-OHB levels decreased glucose concentration. The effects observed were transient and dependent on 3-OHB concentration; these effects may help explain most of the other effects of noradrenaline described here. The role of 3-OHB as a regulator of adrenergic responses seems to be part of a complex fail-safe mechanism which prevents wasting.
Publisher version (URL):http://jeb.biologists.org/content/200/20/2641.abstract
E-ISSNmetadata.dc.identifier.doi = DOI:1477-9145
Appears in Collections:(IIBB) Artículos

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