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Closed Access item Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex

Authors:Amargós-Bosch, Mercè
Bortolozzi, Analía
Puig, M. Victoria
Serrats, Jordi
Adell, Albert
Celada, Pau
Toth, Miklos
Mengod, Guadalupe
Artigas, Francesc
Keywords:5-HT1A receptors, 5-HT 2A receptors, Antipsychotics, Medial prefrontal cortex, Pyramidal neuron
Issue Date:Mar-2004
Publisher:Oxford University Press
Citation:Cerebral Cortex 14(3): 281-299 (2004)
Abstract:The prefrontal cortex plays a key role in the control of higher brain functions and is involved in the pathophysiology and treatment of schizophrenia. Here we report that ∼60% of the neurons in rat and mouse prefrontal cortex express 5-HT1A and/or 5-HT2A receptor mRNAs, which are highly co-localized (∼80%). The electrical stimulation of the dorsal and median raphe nuclei elicited 5-HT1A-mediated inhibitions and 5-HT2A-mediated excitations in identified pyramidal neurons recorded extracellularly in rat medial prefrontal cortex (mPFC). Opposite responses in the same pyramidal neuron could be evoked by stimulating the raphe nuclei at different coordinates, suggesting a precise connectivity between 5-HT neuronal subgroups and 5-HT1A and 5-HT2A receptors in pyramidal neurons. Microdialysis experiments showed that the increase in local 5-HT release evoked by the activation of 5-HT2A receptors in mPFC by DOI (5-HT2A/2C receptor agonist) was reversed by co-perfusion of 5-HT1A agonists. This inhibitory effect was antagonized by WAY-100635 and the prior inactivation of 5-HT1A receptors in rats and was absent in mice lacking 5-HT1A receptors. These observations help to clarify the interactions between the mPFC and the raphe nuclei, two key areas in psychiatric illnesses and improve our understanding of the action of atypical antipsychotics, acting through these 5-HT receptors.
Publisher version (URL):http://dx.doi.org/10.1093/cercor/bhg128
E-ISSNmetadata.dc.identifier.doi = DOI:1460-2199
Appears in Collections:(IIBB) Artículos

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