Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/34464
Share/Export:
![]() ![]() |
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Title: | Lignin composition and structure in young versus adult Eucalyptus globulus plants |
Authors: | Rencoret, Jorge CSIC ORCID; Gutiérrez Suárez, Ana CSIC ORCID ; Nieto, Lidia CSIC; Jiménez-Barbero, Jesús CSIC ORCID; Faulds, Craig B. CSIC; Kim, Hoon; Ralph, John; Martínez, Ángel T. CSIC ORCID ; Río Andrade, José Carlos del CSIC ORCID | Keywords: | Eucalyptus globulus Lignification Milled-wood lignin (MWL) Pyrolysis-GC/MS 2D NMR HSQC Gel-state NMR Thioacidolysis β-O-4′ β-β′ units |
Issue Date: | 2011 | Publisher: | American Society of Plant Biologists | Citation: | Plant Physiology 155, 667-682, 2011 | Abstract: | Lignin changes during plant growth were investigated in a selected Eucalyptus globulus clone. The lignin composition and structure were studied “in situ” by a new procedure enabling the acquisition of 2D-NMR spectra on wood gels formed in the NMR tube, as well as by analytical pyrolysis (Py-GC/MS). In addition, milled-wood lignins were isolated and analyzed by 2D-NMR, Py-GC/MS, and thioacidolysis. The data indicated that p-hydroxyphenyl (H) and guaiacyl (G) units are deposited at the earlier stages, whereas the woods are enriched in syringyl (S) lignin during late lignification. Wood 2D-NMR showed that β-O-4′ and resinol linkages were predominant in the eucalypt lignin, whereas other substructures were present in much lower amounts. Interestingly, open β-1′ structures could be detected in the isolated lignins. Phenylcoumarans and cinnamyl end-groups were depleted with age, while spirodienone abundance increased, and the main substructures (β-O-4′ and resinols) were scarcely modified. Thioacidolysis revealed a higher predominance of S units in the ether-linked lignin than in the total lignin and, in agreement with NMR, also indicated that resinols are the most important non-ether linkages. Dimer analysis showed that most of the resinol-type structures comprised two S units (syringaresinol), the crossed G-S resinol appearing as a minor substructure and pinoresinol being totally absent. Changes in hemicelluloses were also shown by the 2D-NMR spectra of the wood gels, without polysaccharide isolation. These include decreases of methyl galacturonosyl, arabinosyl and galactosyl (anomeric) signals, assigned to pectin and related neutral polysaccharides, and increases of xylosyl (which are ~50% acetylated) and 4-O-methylglucuronosyl signals. | URI: | http://hdl.handle.net/10261/34464 | DOI: | 10.1104/pp.110.167254 |
Appears in Collections: | (IRNAS) Artículos (CIB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2011-Rencoret-PlantPhysiol-155-667.pdf | 778,2 kB | Adobe PDF | ![]() View/Open |
Review this work
PubMed Central
Citations
34
checked on May 9, 2022
SCOPUSTM
Citations
221
checked on May 21, 2022
WEB OF SCIENCETM
Citations
212
checked on May 21, 2022
Page view(s)
422
checked on May 23, 2022
Download(s)
344
checked on May 23, 2022
Google ScholarTM
Check
Altmetric
Dimensions
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.