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http://hdl.handle.net/10261/34405
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Artigas, Francesc | - |
dc.contributor.author | Gelpí, Emili | - |
dc.date.accessioned | 2011-04-07T11:16:21Z | - |
dc.date.available | 2011-04-07T11:16:21Z | - |
dc.date.issued | 1979-01-01 | - |
dc.identifier.citation | Analytical Biochemistry 92 (1) : 233-242 (1979) | es_ES |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | http://hdl.handle.net/10261/34405 | - |
dc.description.abstract | A new method for the concurrent extraction and quantification of tryptophan (Trp), tryptamine (T), indole-3-acetic acid (IAA), serotonin (5-HT), and 5-hydroxyindole-3-acetic acid (5-HIAA) in samples of rat brain is presented. Homogenization is carried out in 0.1 HCl containing 1 KCl and 0.2% NaHSO3. After centrifugation at 100,000g, the supernatant is percolated through a column of XAD-2 resin, eluted with distilled methanol, and the resulting eluate is evaporated to dryness. The dry residue is then derivatized to yield the pentafluoropropionated (PFP) and methylpentafluoropropionated (Me-PFP) derivatives. Identification and quantification is readily achieved by gas chromatography-mass fragmentographic analysis on a OV-17 or Dexsil 300 column. Endogenous levels in whole rat brain established by this method are IAA, 13,1 ± 2.0 ng/g (n = 6); T, less than 380 pg/g (n = 6); Trp, 4.16 ± 0.23 μg/g (n = 6); 5-HIAA, 442 ± 24 ng/g (n = 6); and 5-HT, 526 ± 81 ng/g (n = 5). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | A new mass fragmentographic method for the simultaneous analysis of tryptophan, tryptamine, indole-3-acetic acid, serotonin, and 5-hydroxyindole-3-acetic acid in the same sample of rat brain | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/0003-2697(79)90651-1 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/0003-2697(79)90651-1 | es_ES |
dc.identifier.e-issn | 1096-0309 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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