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Título: | An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene. |
Autor: | Carranco, Raúl CSIC ORCID; Almoguera, Concepción CSIC ORCID ; Jordano, Juan CSIC ORCID | Palabras clave: | Heat shock element Upstream sequences Seed-specific expression Heat shock protein gene Transgenic tobacco Nicotiana tabacum Sunflower Zygotic embryogenesis Seed desiccation Mutagenesis Transgene expression Factor Seeds Gene promoters |
Fecha de publicación: | nov-1999 | Editor: | American Society of Plant Biologists | Citación: | Plant Physiology 121: 723–730 (1999) | Resumen: | Chimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacco (Nicotiana tabacum) its unique seed-specific expression patterns previously reported in sunflower. These constructs did not respond to heat shock, but were expressed without exogenous stress during late zygotic embryogenesis coincident with seed desiccation. Site-directed mutagenesis of its distal and imperfect heat shock element strongly impaired in vitro heat shock transcription factor binding and transgene expression in seeds. Deletion analyses of upstream sequences indicated the contribution of additional cis-acting elements with either positive or negative effects on transgene expression. These results show differences in the transcriptional activation through the heat shock element of small heat shock protein gene promoters in seeds compared with the heat shock response. In addition, they suggest that heat shock transcription factors and other distinct trans-acting factors cooperate in the regulation of Ha hsp17.6 G1 during seed desiccation. | Versión del editor: | http://dx.doi.org/10.1104/pp.121.3.723 | URI: | 10261/3435 | DOI: | 10.1104/pp.121.3.723 | ISBN: | 1532-2548 |
Aparece en las colecciones: | (IRNAS) Artículos |
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