English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/33621
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Autocrine regulation of human sperm motility by tachykinins

AuthorsPinto Pérez, Francisco M. ; Ravina, Cristina G.; Subiran, Nerea; Cejudo-Román, Antonio ; Fernández-Sánchez, Manuel; Irazusta, Jon; Garrido, Nicolás; Candenas, M. Luz
Issue Date26-Aug-2010
PublisherBioMed Central
CitationReproductive Biology and Endocrinology 8: 104 (2010)
Abstract[Background]: We examined the presence and function of tachykinins and the tachykinin-degrading enzymes neprilysin (NEP) and neprilysin-2 (NEP2) in human spermatozoa. [Methods]: Freshly ejaculated semen was collected from forty-eight normozoospermic human donors. We analyzed the expression of substance P, neurokinin A, neurokinin B, hemokinin-1, NEP and NEP2 in sperm cells by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot and immunocytochemistry assays and evaluated the effects of the neprilysin and neprilysin-2 inhibitor phosphoramidon on sperm motility in the absence and presence of tachykinin receptor-selective antagonists. Sperm motility was measured using WHO procedures or computer-assisted sperm analysis (CASA). [Results]: The mRNAs of the genes that encode substance P/neurokinin A (TAC1), neurokinin B (TAC3), hemokinin-1 (TAC4), neprilysin (MME) and neprilysin-2 (MMEL1) were expressed in human sperm. Immunocytochemistry studies revealed that tachykinin and neprilysin proteins were present in spermatozoa and show specific and differential distributions. Phosphoramidon increased sperm progressive motility and its effects were reduced in the presence of the tachykinin receptor antagonists SR140333 (NK1 receptor-selective) and SR48968 (NK2 receptor-selective) but unmodified in the presence of SR142801 (NK3 receptor-selective). [Conclusion]: These data show that tachykinins are present in human spermatozoa and participate in the regulation of sperm motility. Tachykinin activity is regulated, at least in part, by neprilysins.
Description10 páginas, 5 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1186/1477-7827-8-104
Appears in Collections:(IIQ) Artículos
Files in This Item:
File Description SizeFormat 
1477-7827-8-104.pdf1,2 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.