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Title

Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter

AuthorsDíaz-Martín, J. ; Prieto-Dapena, P. ; Jordano, Juan ; Espinosa-Vázquez, José Manuel; Almoguera, Concepción
KeywordsActivator Fuction
Shock Proteins
Expression
Arabidopsis
Embryogenesis
Domain
Identification
Tolerance
Sunflower
Nerwork
Issue Date21-Oct-2005
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 139:1483-1494 (2005)
AbstractHahsp17.6G1 is the promoter of a small heat stress protein (sHSP) from sunflower (Helianthus annuus) that is activated during zygotic embryogenesis, but which does not respond to heat stress. We report here the cloning of a transcription factor (TF), sunflower drought-responsive element binding factor 2 (HaDREB2), by one-hybrid interaction with functional cis-elements in Hahsp17.6G1. We have analyzed the functional interaction between HaDREB2 and a second transcription factor, sunflower heat stress factor A9 (HaHSFA9), which was previously assigned to the regulation of Hahsp17.6G1. HaDREB2 and HaHSFA9 synergistically trans-activate the Hahsp17.6G1 promoter in bombarded sunflower embryos. This synergistic interaction is heat stress factor (HSF) specific and requires the binding of both factors to the promoter. The C-terminal region of HaHSFA9 is sufficient for the HSF specificity. Our results represent an example of a functional interaction between members of the Apetala 2 (HaDREB2) and HSF (HaHSFA9) families of transcription factors. We suggest new roles in zygotic embryogenesis for specific members of the AP2 transcription factor family.
Publisher version (URL)http://www.plantphysiol.org/cgi/reprint/139/3/1483
URIhttp://hdl.handle.net/10261/3347
DOI10.1104/pp.105.069963.
ISBN0032-0889
Appears in Collections:(IRNAS) Artículos
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