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Polyphasic characterization of phosphate-solubilizing bacteria isolated from rhizospheric soil of the north-eastern region of Portugal.
|Authors:||Valverde Portal, Ángel; Igual, José Mariano ; Cervantes, Emilio|
|Keywords:||Phosphate solubilizing bacteria,|
|Citation:||First International Meeting on Microbial Phosphate Solubilization. Developments in Plant and Soil Sciences. Editors E. Velázquez, C. Rodríguez-Barrueco. Volume 102. 2007, p: 273-276|
|Abstract:||Phosphate-solubilizing microorganisms are often used as plant growth promoters. In the frame of a project focused on the isolation of endosymbionts and ectorhizospheric bacteria associated with Phaseolus vulgaris plants growing in a mountain soil at the north-east of Portugal, we obtained six bacterial strains with high phosphate-solubilizing capability, as demonstrated by the formation of ‘halos’ when plated in YED medium supplemented with tricalcium-phosphate. These bacterial strains were characterized by a polyphasic approach using both phenotypic (API 20 NE) and molecular assays (RAPD, with M13 primer, TP-RAPD, and 16S rDNA sequencing). TP-RAPD yielded an identical band patterns in the six strains indicating that they belong to the same bacterial species. The 16S rRNA sequence analysis of a group-representative strain (P4-22) revealed a sequence similarity value of 99.27% with Pseudomonas jessenii. Moreover, the RAPD fingerprints of these six strains did not show differences, indicating that they form a highly homogenous group. This high homogeneity could be a consequence of the recurring agronomical practices used in this region from the antiquity that include organic fertilization and monoculture of Phaseolus vulgaris.|
|Description:||First International Meeting on Microbial Phosphate Solubilization. Salamanca, Spain, 16-19 july 2002. 4 p.|
|Publisher version (URL):||http://www.springerlink.com/content/978-1-4020-4019-1#section=290601&page=3&locus=0|
|Appears in Collections:||(IRNASA) Libros y partes de libros|
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