English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/33249
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

The transmembrane domain of podoplanin is required for its association with lipid rafts and the induction of epithelial-mesenchymal transition

AuthorsFernández-Muñoz, Beatriz ; Yurrita, María M. ; Martín-Villar, Ester ; Carrasco-Ramírez, Patricia ; Megías, Diego; Renart, Jaime ; Quintanilla, Miguel
KeywordsPodoplanin
DRMs
Lipid rafts
Transmembrane domain
GXXXG motif
EMT
Issue Date3-Mar-2011
PublisherElsevier
CitationInternational Journal of Biochemistry and Cell Biology 43(6): 886-896 (2011)
AbstractPodoplanin is a transmembrane glycoprotein that is upregulated in cancer and was reported to induce an epithelial-mesenchymal transition (EMT) in MDCK cells. The promotion of EMT was dependent on podoplanin binding to ERM (ezrin, radixin, moesin) proteins through its cytoplasmic (CT) domain, which led to RhoA-associated kinase (ROCK)-dependent ERM phosphorylation. Using detergent-resistant membrane (DRM) assays, as well as transmembrane (TM) interactions and ganglioside GM1 binding, we present evidence supporting the localization of podoplanin in raft platforms important for cell signalling. Podoplanin mutant constructs harbouring a heterologous TM region or lacking the CT tail were unable to associate with DRMs, stimulate ERM phosphorylation and promote EMT or cell migration. Similar effects were observed upon disruption of a GXXXG motif within the TM domain, which is involved in podoplanin self-assembly. In contrast, deletion of the extracellular (EC) domain did not affect podoplanin DRM association. Together, these data suggest that both the CT and TM domains are required for podoplanin localization in raft platforms, and that this association appears to be necessary for podoplanin-mediated EMT and cell migration.
Publisher version (URL)http://dx.doi.org/10.1016/j.biocel.2011.02.010
URIhttp://hdl.handle.net/10261/33249
DOI10.1016/j.biocel.2011.02.010
ISSN1357-2725
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
The transmembrane domain.pdf1,65 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.