English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3305
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 16 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis

Autor Ocampo-Sosa, Alain Antonio; García-Lobo, Juan M.
Palabras clave Microbiology
Brucella
Genome
Insertion Sequence (IS)
IS711 transposition
Fecha de publicación 24-ene-2008
EditorBioMed Central
Citación BMC Microbiology 8: 17 (2008)
Resumen[Background] The Brucella genome contains an insertion sequence (IS) element called IS711 or IS6501, which is specific to the genus. The copy number of IS711 varies in the genome of the different Brucella species, ranging from 7 in B. abortus, B. melitensis and B. suis to more than 30 in B. ovis and in Brucella strains isolated from marine mammals. At present, there is no experimental evidence of transposition of IS711, but the occurrence of this element with a high copy number in some species, and the isolation of Brucella strains with "ectopic" copies of IS711 suggested that this IS could still transpose.
[Results] In this study we obtained evidence of transposition of IS711 from the B. ovis and B. pinnipedialis chromosomes by using the "transposon trap" plasmid pGBG1. This plasmid expresses resistance to tetracycline only if the repressor gene that it contains is inactivated. The strains B. melitensis 16 M, B. abortus RB51, B. ovis BOC22 (field strain) and B. pinnipedialis B2/94, all containing the plasmid pGBG1, were grown in culture media with tetracycline until the appearance of tetracycline resistant mutants (TcR). TcR mutants due to IS711 transposition were only detected in B. ovis and B. pinnipedialis strains.
[Conclusion] Four different copies of IS711 were found to transpose to the same target sequence in the plasmid pGBG1. This demonstrated that IS711 are active in vivo, specially in Brucella species with a high number of IS711 copies as B. ovis and B. pinnipedialis.
Descripción The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2180/8/17
URI http://hdl.handle.net/10261/3305
DOI10.1186/1471-2180-8-17
ISSN1471-2180
Aparece en las colecciones: (IBBTEC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Demonstration of IS711.pdf812,23 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.