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Título: | An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination |
Autor: | Barral, Patricia; Suárez, Cynthia CSIC; Batanero, Eva; Alfonso, Carlos CSIC ORCID ; Alché Ramírez, Juan de Dios CSIC ORCID; Rodríguez García, María I. CSIC ORCID ; Villalba, Mayte; Rivas, Germán CSIC ORCID CVN ; Rodríguez, Rosalía | Palabras clave: | Allergens Carbohydrate-binding module 1,3-b-glucan Olive pollen Pollen germination |
Fecha de publicación: | ago-2005 | Editor: | Portland Press | Citación: | Biochemical Journal 15(1): 77-84 (2005) | Resumen: | CBMs (carbohydrate-binding modules) are the most common non-catalytic modules associated with enzymes active in plant cell-wall hydrolysis. They have been frequently identified by amino acid sequence alignments, but only a few have been experimentally established to have a carbohydrate-binding activity. A small olive pollen protein, Ole e 10 (10 kDa), has been described as a major inducer of type I allergy in humans. In the present study, the ability of Ole e 10 to bind several polysaccharides has been analysed by affinity gel electrophoresis, which demonstrated that the protein bound 1,3-b-glucans preferentially. Analytical ultracentrifugation studies confirmed binding to laminarin, at a protein/ligand ratio of 1:1. The interaction of Ole e 10 with laminarin induced a conformational change in the protein, as detected by CD and fluorescence analyses, and an increase of 3.6 °C in the thermal denaturation temperature of Ole e 10 in the presence of the glycan. These results, and the absence of alignment of the sequence of Ole e 10 with that of any classified CBM, indicate that this pollen protein defines a novel family of CBMs, which we propose to name CBM43. Immunolocalization of Ole e 10 in mature and germinating pollen by transmission electron microscopy and confocal laser scanning microscopy demonstrated the co-localization of Ole e 10 and callose (1,3-b-glucan) in the growing pollen tube, suggesting a role for this protein in the metabolism of carbohydrates and in pollen tube wall re-formation during germination. | Descripción: | 8 páginas, 8 figuras, 2 tablas. | Versión del editor: | http://dx.doi.org/10.1042/BJ20050456 | URI: | http://hdl.handle.net/10261/32735 | DOI: | 10.1042/BJ20050456 | ISSN: | 0264-6021 |
Aparece en las colecciones: | (EEZ) Artículos (CIB) Artículos |
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