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dc.contributor.authorAlché Ramírez, Juan de Dios-
dc.contributor.authorCastro López, Antonio Jesús-
dc.contributor.authorRodríguez García, María I.-
dc.date.accessioned2011-02-23T11:38:18Z-
dc.date.available2011-02-23T11:38:18Z-
dc.date.issued2002-
dc.identifier.citationMicron 33(1): 33-37 (2002)es_ES
dc.identifier.issn0968-4328-
dc.identifier.urihttp://hdl.handle.net/10261/32729-
dc.description5 áginas, 1 figura.es_ES
dc.description.abstractIn situ reverse transcription-PCR of mRNAs corresponding to the olive major allergen (Ole e I) has been tested at the ultrastructural level in mature olive pollen. The transcripts were present in the cytoplasm of both the vegetative and the generative cells, frequently associated to ribosomes in the endoplasmic reticulum. No labeling was detected in the pollen wall, nor in vacuoles, lipid bodies, plastids or mitochondria. Localization of the major olive allergen at ultrastructural level showed the protein present mainly in the lumen of the endoplasmic reticulum vesicles or pockets scattered in the cytoplasm, and in the outer region of the pollen exine. The results confirm the rough endoplasmic reticulum as the cell system involved in both the synthesis and storage of this protein. This is the first report of in situ RT-PCR on plant material at the ultrastructural level. The method described for mRNA amplification and detection is confirmed as a valuable tool for studying gene expression in plant material.es_ES
dc.description.sponsorshipThis work was supported by CICYT (Spanish MEC) Project OLI1996-2160.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectOlivees_ES
dc.subjectOlea europaeaes_ES
dc.subjectPollenes_ES
dc.subjectAllergenes_ES
dc.subjectIn situ RT-PCRes_ES
dc.subjectOle e Ies_ES
dc.titleLocalization of transcripts corresponding to the major allergen from olive pollen (Ole e I) by electron microscopic non-radioactive in situ RT-PCRes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/S0968-4328(00)00074-3-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/S0968-4328(00)00074-3es_ES
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