English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32725
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER

AuthorsAlché Ramírez, Juan de Dios; Castro López, Antonio Jesús; Olmedilla, Adela; Fernández, Mari Carmen; Rodríguez, Rosalía; Villalba, Mayte; Rodríguez García, María I.
KeywordsOle e I
Allergen
Pollen
Tapetum
Expression
Olea europaea L.
Issue Date1999
PublisherCompany of Biologists
CitationJournal of Cell Science 112(15): 2501-2509 (1999)
AbstractThe distribution of Ole e I (the major olive pollen allergen) and its transcripts was investigated in the anther from premeiotic stages until the dehiscent pollen stage. Crude protein extracts were analyzed by immunoblotting and probed with a monoclonal antibody to Ole e I. The protein, with three variants, was found to accumulate from the early microspore stage onwards. In addition to the previously reported localization of the protein, Ole e I has been immunolocalized for the first time within the pollen wall and in the tapetum. Reverse transcription-polymerase chain reaction analysis using specific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards. No expression was found in control tissues such as petals, roots or leaves. Light microscopy in situ hybridization on developing flower buds and dehiscent pollen confirmed the transcripts to be present in both the microspores and the sporophytic tissue (tapetum). Labeling was found primarily in the tapetum, reaching the highest concentration in the cytoplasm of the developing and mature pollen, once tapetum started to degenerate. In situ hybridization at the transmission electron microscope level showed the transcripts to accumulate on ribosomes of the rough endoplasmic reticulum. These studies, together with others carried out previously by us, indicated that both synthesis and storage of Ole e I take place in the endoplasmic reticulum, coincidentally with the conspicuous changes suffered by this membrane system during pollen development. This process is most likely controlled at the transcriptional level. The localization of the protein in the pollen ectexine bring new insights into the function of the allergen, which are discussed.
Description9 páginas, 5 figuras.
Publisher version (URL)http://jcs.biologists.org/cgi/content/abstract/112/15/2501
URIhttp://hdl.handle.net/10261/32725
ISSN0021-9533
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
2501.pdf536,72 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.