Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/32701
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Alché Ramírez, Juan de Dios | - |
dc.contributor.author | Dickinson, Hugh | - |
dc.date.accessioned | 2011-02-22T14:25:48Z | - |
dc.date.available | 2011-02-22T14:25:48Z | - |
dc.date.issued | 1998-02 | - |
dc.identifier.citation | Protein Expression and Purification 12(1): 138-143 (1998) | es_ES |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | http://hdl.handle.net/10261/32701 | - |
dc.description | 6 páginas, 4 figuras, 1 tabla. | es_ES |
dc.description.abstract | HOP1,a protein component of the synaptonemal complex inSaccharomyces cerevisiaewhich is believed to play an important role in meiotic synapsis, was expressed inEscherichia colias a fusion protein incorporating a “tag” polypeptide which is biotinylated naturally in the bacteria. TheHOP1fusion protein was produced in an insoluble form within the bacteria; once solubilized using a denaturing agent, the protein was purified by avidin monomer affinity chromatography. The recombinant protein was used to immunize rabbits and produce polyclonal antibodies. Procedures for affinity purification of antibodies using the recombinant protein attached to the avidin column and a magnetic method for concentration of antibodies are described. Antibody elution conditions in these procedures do not affect the affinity of the column for the recombinant protein, which can be recovered afterward. Affinity-purified antibodies show high binding capacity toHOP1recombinant protein in immunoblotting experiments, but reduced background compared with crude antiserum or purified IgG fraction. The affinity-purified antibodies recognize a major band around 70 kDa in Western blots of yeast protein extracts following meiotic induction. | es_ES |
dc.description.sponsorship | This work was funded by European Commission HCM Programme, proposal ERB4001GT921419, and by Spanish MEC (Subprograma de Perfeccionamineto para Doctores y Tecnólogos en el Extranjero). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Chromatography | es_ES |
dc.subject | Affinity purification | es_ES |
dc.subject | HOP1 | es_ES |
dc.subject | Fusion protein | es_ES |
dc.subject | Yeast | es_ES |
dc.subject | Antibodies | es_ES |
dc.title | Affinity Chromatographic Purification of Antibodies to a Biotinylated Fusion Protein Expressed inEscherichia coli | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1006/prep.1997.0824 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1006/prep.1997.0824 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (EEZ) Artículos |
CORE Recommender
SCOPUSTM
Citations
10
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
5
checked on 24-feb-2024
Page view(s)
393
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.