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Título: | Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment |
Autor: | Carrascal, Montserrat CSIC ORCID; Ovelleiro, David; Casas, Vanessa CSIC; Gay, Marina CSIC ORCID; Abián, Joaquín CSIC ORCID | Palabras clave: | Phosphoproteomics T lymphocytes Immune response IMAC Titanium oxide LC−MSn LymPHOS database |
Fecha de publicación: | 6-nov-2008 | Editor: | American Chemical Society | Citación: | Journal of Proteome Research 7(12): 5167–5176 (2008) | Resumen: | T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (http://lymphos.org), which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences. | Versión del editor: | http://dx.doi.org/10.1021/pr800500r | URI: | http://hdl.handle.net/10261/32522 | DOI: | 10.1021/pr800500r | ISSN: | 1535-3893 | E-ISSN: | 1535-3907 |
Aparece en las colecciones: | (IIBB) Artículos |
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