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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32522
Title: Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment
Authors: Carrascal, Montserrat; Ovelleiro, David; Casas, Vanessa; Gay, Marina; Abián, Joaquín
Keywords: Phosphoproteomics
T lymphocytes
Immune response
Titanium oxide
LymPHOS database
Issue Date: 6-Nov-2008
Publisher: American Chemical Society
Citation: Journal of Proteome Research 7(12): 5167–5176 (2008)
Abstract: T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (http://lymphos.org), which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences.
Publisher version (URL): http://dx.doi.org/10.1021/pr800500r
URI: http://hdl.handle.net/10261/32522
ISSN: 1535-3893
DOI: 10.1021/pr800500r
E-ISSN: 1535-3907
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