Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32502
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Title

Spermine regulates N-methyl-D -aspartate receptor desensitization

AuthorsLerma Gómez, Juan
Issue DateFeb-1992
PublisherElsevier
CitationNeuron 8(2): 343-352 (1992)
AbstractThe action of the endogenous polyamine spermine on NMDA-induced responses (in the presence of glycine) was evaluated in cultured spinal cord neurons under voltage- and concentration-clamp conditions. Spermine potentiated NMDA-induced responses in a dose-dependent manner. It was more effective in potentiating steadystate currents (i.e., desensitized response) than the peak phase of the response, indicating that the degree of desensitization was reduced in the presence of the polyamine. Kinetic analysis revealed that the desensitization onset rate, but not recovery rate, was affected by spermine. The effect was voltage independent and was seen in thoroughly dialyzed cells, in which desensitization becomes independent of glycine. Spermine potentiation showed fast on-off kinetics, and intracellular spermine, loaded in the recording pipette, did not occlude potentiation by extracellularly applied spermine. These results are consistent with the existence of a modulatory site for polyamines in the extracellular domain of the NMDA receptor, the activation of which potentiates NMDA receptor function by regulating its desensitization kinetics.
Description10 páginas, 9 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1016/0896-6273(92)90300-3
URIhttp://hdl.handle.net/10261/32502
DOI10.1016/0896-6273(92)90300-3
ISSN0896-6273
Appears in Collections:(IC) Artículos

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