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Título

A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant cKi-ras allele in human tumor cells

AutorWinter, E.; Yamamoto, Fumiko; Almoguera, Concepción ; Perucho, M.
Palabras claveHuman tumors
Activated ras oncogenes
Mutations
Condo
Pancreatic
RNA
C-ki-ras genes
Tumor cells
Fecha de publicación1985
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences of the USA Vol. 82, Issue 22, 7575-7579, (1985)
ResumenA significant percentage of human tumors contain activated ras oncogenes that have acquired oncogenic potential as a result of somatic point mutations at codon 12 or 61 of the encoded ras gene product. We report here a method to detect and characterize mutations in ras genes that is based on the ability of pancreatic ribonuclease (RNase A; EC 3.1.27.5) to cleave RNA heteroduplexes containing single-base mismatches. Using this method, we show that certain human tumor cells contain mutant c-Ki-ras genes, and we define the nature and position of these mutations. At the same time, we describe the presence and estimate the expression of both normal and mutant c-Ki-ras alleles in the same tumor cells. This method should be useful for the diagnostic detection and characterization of single point mutations in expressed genes.
Versión del editorhttp://www.pnas.org/content/82/22/7575.full.pdf
URIhttp://hdl.handle.net/10261/32456
ISSN0027-8424
E-ISSN1091-6490
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