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Title

Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes

AuthorsAlmoguera, Concepción ; Shibata, D.; Forrester, K.; Martin, J.; Amheim, N.; Perucho, M.
KeywordsDNA
Pancreatic ribonuclease
RNA
Adenocarcinoma
Genetics
Gene amplification
Cell culture
Cell line
Codon
Colon tumor
Gallbladder tumor
Fibroblast
Human
Mutation
Nucleic acid hybridization
Oncogene ras
Paget nipple disease
Pancreas tumor
Pathology
Issue Date1988
PublisherElsevier
CitationCell 53 (4): 549-554, (1988)
AbstractUsing in vitro gene amplification by the polymerase chain reaction (PCR) and mutation detection by the RNAase A mismatch cleavage method, we have examined, c-K-ras genes in human pancreatic carcinomas. We used frozen tumor specimens and single 5 μm sections from formalin-fixed, paraffin-embedded tumor tissue surgically removed or obtained at autopsy. Twenty-one out of 22 carcinmas of the exocrine pancreas contained c-K-ras genes with mutations at codon 12. In seven cases tested, the mutation was present in both primary tumors and their corresponding metastases. from the same cancer patients or in five gall bladder carcinomas. We conclude from these results that c-K-ras somatic mutational activation is a critical event in the oncogenesis of most, if not all, human cancers of the exocrine pancreas.
Publisher version (URL)http://www.sciencedirect.com/science/article/B6WSN-4D5DY1T-9/2/1ef0c1534ac86b61fb8b62b823ab0867
URIhttp://hdl.handle.net/10261/32452
DOI10.1016/0092-8674(88)90571-5
ISSN0092-8674
E-ISSN1097-4172
Appears in Collections:(IRNAS) Artículos
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