English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32352
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorCasanovas, Albert-
dc.contributor.authorCarrascal, Montserrat-
dc.contributor.authorAbián, Joaquín-
dc.contributor.authorLópez-Tejero, M. Dolores-
dc.contributor.authorLlobera, Miquel-
dc.date.accessioned2011-02-15T09:21:00Z-
dc.date.available2011-02-15T09:21:00Z-
dc.date.issued2008-07-17-
dc.identifier.citationJournal of Proteome Research 7(9): 4173–4177 (2008)es_ES
dc.identifier.issn1535-3893-
dc.identifier.urihttp://hdl.handle.net/10261/32352-
dc.description.abstractSpecific antibodies are essential tools for studying proteins. P66 is a chicken polyclonal antibody raised against bovine lipoprotein lipase (LPL) that has been used in earlier studies. Here, we developed a two-dimensional (2D) Western blot with reducing gels, using commercial bovine LPL (53 kDa) as a standard and P66 for detection. Our results revealed incomplete purification of commercial LPL and nonspecificity of P66, both undetectable in one-dimensional analysis. Antithrombin III (ATIII) was identified as both a major contaminant in commercial LPL and a cross-reacting protein with P66. Although LPL purification methods were presumably designed to eliminate ATIII, here we demonstrate that some procedures fell short of this objective and thus led to the production of a nonspecific antibody. Our results define 2D electrophoresis/Western blot and mass spectrometric protein identification as the most reliable procedure for validating LPL purity and the specificity of antibodies against this enzyme.es_ES
dc.description.sponsorshipThis study was funded by grants SAF2005-02391 and BFU2007-65247/BMC from the Dirección General de Investigación Científica y Técnica, Spanish Ministry of Education and Science. Albert Casanovas was the recipient of a fellowship from the Ministry of Universities, Research and Information Society of the Generalitat de Catalunya and from European Social Funds.-
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsclosedAccesses_ES
dc.subjectTwo-dimensional Western blotes_ES
dc.subjectAntithrombin IIIes_ES
dc.subjectBovinees_ES
dc.subjectP66es_ES
dc.subject5D2es_ES
dc.titleApplication of proteomic tools to detect the nonspecificity of a polyclonal antibody against lipoprotein lipasees_ES
dc.typeArtículoes_ES
dc.identifier.doi10.1021/pr800131n-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://pubs.acs.org/doi/abs/10.1021/pr800131nes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1021/pr800131n-
dc.identifier.e-issn1535-3907-
Appears in Collections:(IIBB) Artículos
Files in This Item:
There are no files associated with this item.
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.