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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/32222
Title: Disassembly and reconstitution of yeast 60S ribosomal subunits
Authors: Nieto, M. Ángela; Hernández Torres, Francisco; Palacián, Enrique
Keywords: Yeast ribosomes
Ribosome disassembly
Ribosome reconstitution
Dimethylmaleic anhydride
Issue Date: 1989
Publisher: Springer
Citation: Molecular and Cellular Biochemistry 86(1): 55-63 (1989)
Abstract: Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.
Description: 9 páginas, 6 figuras.
Publisher version (URL): http://dx.doi.org/10.1007/BF00231689
URI: http://hdl.handle.net/10261/32222
ISSN: 0300-8177
DOI: 10.1007/BF00231689
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