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|Title:||Disassembly and reconstitution of yeast 60S ribosomal subunits|
|Authors:||Nieto, M. Ángela; Hernández Torres, Francisco; Palacián, Enrique|
|Citation:||Molecular and Cellular Biochemistry 86(1): 55-63 (1989)|
|Abstract:||Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.|
|Description:||9 páginas, 6 figuras.|
|Publisher version (URL):||http://dx.doi.org/10.1007/BF00231689|
|Appears in Collections:||(CBM) Artículos|
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