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Closed Access item Disassembly and reconstitution of yeast 60S ribosomal subunits

Authors:Nieto, M. Ángela
Hernández, Francisco
Palacián, Enrique
Keywords:Yeast ribosomes, Ribosome disassembly, Ribosome reconstitution, Dimethylmaleic anhydride
Issue Date:1989
Publisher:Springer
Citation:Molecular and Cellular Biochemistry 86(1): 55-63 (1989)
Abstract:Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.
Description:9 páginas, 6 figuras.
Publisher version (URL):http://dx.doi.org/10.1007/BF00231689
URI:http://hdl.handle.net/10261/32222
ISSN:0300-8177
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Appears in Collections:(CBM) Artículos

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