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Título : Disassembly and reconstitution of yeast 60S ribosomal subunits
Autor : Nieto, M. Ángela, Hernández, Francisco, Palacián, Enrique
Palabras clave : Yeast ribosomes
Ribosome disassembly
Ribosome reconstitution
Dimethylmaleic anhydride
Fecha de publicación : 1989
Editor: Springer
Citación : Molecular and Cellular Biochemistry 86(1): 55-63 (1989)
Resumen: Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.
Descripción : 9 páginas, 6 figuras.
Versión del editor:
ISSN: 0300-8177
DOI: 10.1007/BF00231689
Citación : Molecular and Cellular Biochemistry 86(1): 55-63 (1989)
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