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Title

Genetic analysis of the presumptive blood from Louis XVI, king of France

AuthorsLalueza-Fox, Carles ; Gigli, Elena ; Binid, Carla; Calafell, Francesc ; Luiselli, Donata; Pelotti, Susi; Pettener, Davide
KeywordsLouis XVI
Identification
Ancient DNA
Mitochondrial DNA
Y-chromosome
Eye colour
Issue DateNov-2011
PublisherElsevier
CitationForensic Science International: Genetics 5(5): 459–463 (2011)
AbstractA text on a pyrographically decorated gourd dated to 1793 explains that it contains a handkerchief dipped with the blood of Louis XVI, king of France, after his execution. Biochemical analyses confirmed that the material contained within the gourd was blood. The mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1) and 2 (HVR2), the Y-chromosome STR profile, some autosomal STR markers and a SNP in HERC2 gene associated to blue eyes, were retrieved, and some results independently replicated in two different laboratories. The uncommon mtDNA sequence retrieved can be attributed to a N1b haplotype, while the novel Y-chromosome haplotype belongs to haplogroup G2a. The HERC2 gene showed that the subject analyzed was a heterozygote, which is compatible with a blue-eyed person, as king Louis XVI was. To confirm the identity of the subject, an analysis of the dried heart of his son, Louis XVII, could be undertaken.
Publisher version (URL)http://dx.doi.org/10.1016/j.fsigen.2010.09.007
URIhttp://hdl.handle.net/10261/32188
DOI10.1016/j.fsigen.2010.09.007
ISSN1875-1768
Appears in Collections:(IBE) Artículos
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