English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31911
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Induction of the Hahsp17.7G4 promoter by root-knot nematodes: involvement of heat-shock elements in promoter activity in giant cells

AuthorsEscobar, Carolina; Barcala, Marta; Portillo, Mary; Almoguera, Concepción ; Jordano, Juan ; Fenoll, Carmen
Feeding structures
Developmental regulation
Seed Maturation
Issue DateDec-2003
PublisherAmerican Phytopathological Society
CitationMPMI - Molecular Plant Microbe Interactions vol.16 núm.12 pág.:1062 -1068, (2003)
AbstractRoot-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita., In roots containing a GUS reporter fusion to the Hahsp17.7G4 promoter, 10% of the galls stained for GUS expression 1 to 3 days after infection and the fraction stained increased to 60 to 80% 17 to 20 days after infection. A DNA fragment from -83 to +163, which contains heat-shock element (HSE) core sequences, is sufficient to support a promoter activity largely restricted to giant cells within the galls. Two-point mutations in HSE cores, previously reported to abolish the heat-shock response and to strongly reduce the embryogenesis response of the same promoter, did not reduce expression in giant cells. This suggests a distinct regulation of the promoter by nematodes. However, additional point mutations located at positions crucial for binding of heat-shock transcription factors (HSFs) caused a severe decrease in the nematode response. These results demonstrate that HSEs are involved in the promoter activation in giant cells and suggest that HSFs may mediate this response.
Publisher version (URL)http://apsjournals.apsnet.org/doi/pdfplus/10.1094/MPMI.2003.16.12.1062
Appears in Collections:(IRNAS) Artículos
Files in This Item:
File Description SizeFormat 
Embargo_Gral.pdf21,67 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.