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dc.contributor.authorCarrascosa, Laura G.-
dc.contributor.authorMartínez-Orellana, Lidia-
dc.contributor.authorHuttel, Yves-
dc.contributor.authorRomán García, Elisa Leonor-
dc.contributor.authorLechuga, Laura M.-
dc.date.accessioned2011-01-21T10:54:13Z-
dc.date.available2011-01-21T10:54:13Z-
dc.date.issued2010-04-
dc.identifier.citationEuropean Biophysics Journal 39(10): 1433-1444 (2010)es_ES
dc.identifier.issn0175-7571-
dc.identifier.urihttp://hdl.handle.net/10261/31416-
dc.description12 páginas, 4 figuras, 1 tabla.es_ES
dc.description.abstractA detailed study of the immobilization of three differently sulfur-modified DNA receptors for biosensing applications is presented. The three receptors are DNA-(CH)n-SH-, DNA-(CH)n-SS-(CH)n-DNA, and DNA-(CH)n-SS-DMTO. Nanomechanical and surface plasmon resonance biosensors and fluorescence and radiolabelling techniques were used for the experimental evaluation. The results highlight the critical role of sulfur linker type in DNA self-assembly, affecting the kinetic adsorption and spatial distribution of DNA chains within the monolayer and the extent of chemisorption and physisorption. A spacer (mercaptohexanol, MCH) is used to evaluate the relative efficiencies of chemisorption of the three receptors by analysing the extent to which MCH can remove physisorbed molecules from each type of monolayer. It is demonstrated that –SH derivatization is the most suitable for biosensing purposes as it results in densely packed monolayers with the lowest ratio of physisorbed probes.es_ES
dc.description.sponsorshipL.M., Y.H., and E.R. acknowledge the Nanoselect Project (CSD2007-00041) from the Spanish Ministry for Innovation and Science. L.G.C. and L.M.L. acknowledge funding from Fundation Botin.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightsclosedAccesses_ES
dc.subjectThiolated DNAes_ES
dc.subjectBiofunctionalizationes_ES
dc.subjectSurface plasmon resonance biosensores_ES
dc.subjectNanomechanical biosensores_ES
dc.subjectFluorescencees_ES
dc.subjectXPSes_ES
dc.titleUnderstanding the role of thiol and disulfide self-assembled DNA receptor monolayers for biosensing applicationses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/s00249-010-0599-6-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s00249-010-0599-6es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderFundación Botín-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100006373es_ES
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