Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/31414
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Treviño, Juan | - |
dc.contributor.author | Calle Martín, Ana | - |
dc.contributor.author | Rodríguez-Frade, José Miguel | - |
dc.contributor.author | Mellado, Mario | - |
dc.contributor.author | Lechuga, Laura M. | - |
dc.date.accessioned | 2011-01-21T10:21:28Z | - |
dc.date.available | 2011-01-21T10:21:28Z | - |
dc.date.issued | 2009-08-11 | - |
dc.identifier.citation | Analytica Chimica Acta 647(2): 202-209 (2009) | es_ES |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/10261/31414 | - |
dc.description | 8 páginas, 3 figuras, 4 tablas. | es_ES |
dc.description.abstract | Single- and multi-analyte detection of two gonadotropic hormones (follicle stimulating hormone (hFSH) and luteinizing hormone (hLH)) was achieved by a Surface Plasmon Resonance (SPR) immunoassay on untreated human urine samples. Multi-analyte detection was accomplished using two alternative formats which are based in the individual or simultaneous immobilization of the hormones on the sensor surface. The lowest detection limit for both hormones in urine was found to be 1 ng mL−1, which in international units (IU) in terms of the World Health Organization (WHO) standards represents 8 mIU mL−1 of hLH and 14 mIU mL−1 of hFSH, respectively. The reliability of the assay was demonstrated by intra- and inter-assay variabilities <6%, chip-to-chip variabilities <5%, recoveries in the range of 80–120% and stability of the sensor response through more than 100 measurements. The sensitivity of this biosensing methodology renders it in a useful technique for the diagnosis of reproductive disorders, as well as for fertility monitoring. | es_ES |
dc.description.sponsorship | The authors gratefully acknowledge the financial support from Fundació La Marató de TV3 and Fundación M. Botín. The Department of Immunology and Oncology was founded and is supported by the Spanish National Research Council (CSIC) and by Pfizer. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | SPR | es_ES |
dc.subject | Immunosensor | es_ES |
dc.subject | Self-assembled monolayer | es_ES |
dc.subject | Human follicle stimulating hormone | es_ES |
dc.subject | Human luteinizing hormone | es_ES |
dc.subject | Point-of-care device | es_ES |
dc.subject | Urine samples | es_ES |
dc.title | Single- and multi-analyte determination of gonadotropic hormones in urine by Surface Plasmon Resonance immunoassay | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.aca.2009.06.002 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.aca.2009.06.002 | es_ES |
dc.contributor.funder | Fundació La Marató de TV3 | - |
dc.contributor.funder | Fundación Botín | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Pfizer | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100008666 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006373 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100004319 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIN2) Artículos (CNB) Artículos (IMN-CNM) Artículos |
CORE Recommender
SCOPUSTM
Citations
22
checked on 10-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 24-feb-2024
Page view(s)
306
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.