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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31414
Title: Single- and multi-analyte determination of gonadotropic hormones in urine by Surface Plasmon Resonance immunoassay
Authors: Treviño, Juan; Calle Martín, Ana ; Rodríguez Frade, José M.; Mellado, Mario; Lechuga, Laura M.
Keywords: SPR
Self-assembled monolayer
Human follicle stimulating hormone
Human luteinizing hormone
Point-of-care device
Urine samples
Issue Date: 11-Aug-2009
Publisher: Elsevier
Citation: Analytica Chimica Acta 647(2): 202-209 (2009)
Abstract: Single- and multi-analyte detection of two gonadotropic hormones (follicle stimulating hormone (hFSH) and luteinizing hormone (hLH)) was achieved by a Surface Plasmon Resonance (SPR) immunoassay on untreated human urine samples. Multi-analyte detection was accomplished using two alternative formats which are based in the individual or simultaneous immobilization of the hormones on the sensor surface. The lowest detection limit for both hormones in urine was found to be 1 ng mL−1, which in international units (IU) in terms of the World Health Organization (WHO) standards represents 8 mIU mL−1 of hLH and 14 mIU mL−1 of hFSH, respectively. The reliability of the assay was demonstrated by intra- and inter-assay variabilities <6%, chip-to-chip variabilities <5%, recoveries in the range of 80–120% and stability of the sensor response through more than 100 measurements. The sensitivity of this biosensing methodology renders it in a useful technique for the diagnosis of reproductive disorders, as well as for fertility monitoring.
Description: 8 páginas, 3 figuras, 4 tablas.
Publisher version (URL): http://dx.doi.org/10.1016/j.aca.2009.06.002
URI: http://hdl.handle.net/10261/31414
DOI: 10.1016/j.aca.2009.06.002
ISSN: 0003-2670
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