Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31413
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Título : Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples
Autor : Treviño, Juan, Calle Martín, Ana, Rodríguez Frade, José M., Mellado, Mario, Lechuga, Laura M.
Palabras clave : SPR
Immunosensor
hTSH
hGH
hFSH
hLH
Fecha de publicación : May-2009
Editor: Elsevier
Citación : Clinica Chimica Acta 403(1-2): 56-62 (2009)
Resumen: Background: Direct determination of four pituitary peptide hormones: human thyroid stimulating hormone (hTSH), growth hormone (hGH), follicle stimulating hormone (hFSH), and luteinizing hormone (hLH) has been carried out using a portable surface plasmon resonance (SPR) immunosensor. Methods: A commercial SPR biosensor was employed. The immobilization of the hormones was optimized and monoclonal antibodies were selected in order to obtain the best sensor performance. Assay parameters as running buffer and regeneration solution composition or antibody concentration were adjusted to achieve a sensitive analyte detection. Results: The performance of the assays was assessed in buffer solution, serum and urine, showing sensitivity in the range from 1 to 6 ng/mL. The covalent attachment of the hormones ensured the stability of the SPR signal through repeated use in up to 100 consecutive assay cycles. Mean intra- and inter-day coefficients of variation were all < 7%, while batch-assay variability using different sensor surfaces was < 5%. Conclusions: Taking account both the excellent reutilization performance and the outstanding reproducibility, this SPR immunoassay method turns on a highly reliable tool for endocrine monitoring in laboratory and point-of-care (POC) settings.
Descripción : 7 páginas, 5 figuras, 1 tabla.
Versión del editor: http://dx.doi.org/10.1016/j.cca.2009.01.015
URI : http://hdl.handle.net/10261/31413
ISSN: 0009-8981
DOI: 10.1016/j.cca.2009.01.015
Appears in Collections:(CIN2) Artículos
(IMM-CNM) Artículos
(CNB) Artículos

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