Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31413
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples

AuthorsTreviño, Juan; Calle Martín, Ana CSIC; Rodríguez Frade, José Miguel; Mellado, Mario ; Lechuga, Laura M. CSIC ORCID
KeywordsSPR
Immunosensor
hTSH
hGH
hFSH
hLH
Issue DateMay-2009
PublisherElsevier
CitationClinica Chimica Acta 403(1-2): 56-62 (2009)
AbstractBackground: Direct determination of four pituitary peptide hormones: human thyroid stimulating hormone (hTSH), growth hormone (hGH), follicle stimulating hormone (hFSH), and luteinizing hormone (hLH) has been carried out using a portable surface plasmon resonance (SPR) immunosensor. Methods: A commercial SPR biosensor was employed. The immobilization of the hormones was optimized and monoclonal antibodies were selected in order to obtain the best sensor performance. Assay parameters as running buffer and regeneration solution composition or antibody concentration were adjusted to achieve a sensitive analyte detection. Results: The performance of the assays was assessed in buffer solution, serum and urine, showing sensitivity in the range from 1 to 6 ng/mL. The covalent attachment of the hormones ensured the stability of the SPR signal through repeated use in up to 100 consecutive assay cycles. Mean intra- and inter-day coefficients of variation were all < 7%, while batch-assay variability using different sensor surfaces was < 5%. Conclusions: Taking account both the excellent reutilization performance and the outstanding reproducibility, this SPR immunoassay method turns on a highly reliable tool for endocrine monitoring in laboratory and point-of-care (POC) settings.
Description7 páginas, 5 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1016/j.cca.2009.01.015
URIhttp://hdl.handle.net/10261/31413
DOI10.1016/j.cca.2009.01.015
ISSN0009-8981
Appears in Collections:(CIN2) Artículos
(IMN-CNM) Artículos
(CNB) Artículos

Show full item record
Review this work

SCOPUSTM   
Citations

53
checked on Jun 30, 2022

WEB OF SCIENCETM
Citations

45
checked on Jul 3, 2022

Page view(s)

331
checked on Jul 7, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.