Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/31410
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Título : Determination of human growth hormone in human serum samples by surface plasmon resonance immunoassay
Autor : Treviño, Juan, Calle Martín, Ana, Rodríguez Frade, José M., Mellado, Mario, Lechuga, Laura M.
Palabras clave : SPR
Immunosensor
Self-assembled monolayer
hGH
Point-of-care device
Serum
Fecha de publicación : 15-May-2009
Editor: Elsevier
Resumen: A surface plasmon resonance immunoassay has been developed to determine human growth hormone (hGH) directly and without pre-treatment in human serum samples. A binding inhibition immunoassay was employed. Antibody concentration, assay buffer and regeneration solution have been optimized in order to reach the best performance and the lower non-specific binding of the matrix components to the sensor surface. The lowest detection limit was 6 ng/mL, with a working range covering the physiological range. Reproducibility of the assay was excellent with both intra-assay and inter-assay relative standard deviations <5%, while a variation of 2.19% was obtained employing different sensor chips. Reutilization of the sensor surface allows its continuous use over 50 measurements with a signal drop <20%. The SPR immunoassay results were validated using enzyme-linked immunosorbent assay (ELISA) showing an excellent correlation (R2 = 0.985). A portable and fully automated system (Sensia SL) was employed in this work. This is the first SPR biosensor assay capable of detecting relevant concentrations of a clinical analyte in serum. This study shows the potentials of this device as a diagnostic tool for the detection of multiple clinical analytes.
Descripción : 6 páginas, 4 figuras, 1 tabla.
Versión del editor: http://dx.doi.org/10.1016/j.talanta.2009.01.012
URI : http://hdl.handle.net/10261/31410
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2009.01.012
Citación : Talanta 78(3): 1011-1016 (2009)
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