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Closed Access item Determination of human growth hormone in human serum samples by surface plasmon resonance immunoassay

Authors:Treviño, Juan
Calle Martín, Ana
Rodríguez Frade, José M.
Mellado, Mario
Lechuga, Laura M.
Keywords:SPR, Immunosensor, Self-assembled monolayer, hGH, Point-of-care device, Serum
Issue Date:15-May-2009
Citation:Talanta 78(3): 1011-1016 (2009)
Abstract:A surface plasmon resonance immunoassay has been developed to determine human growth hormone (hGH) directly and without pre-treatment in human serum samples. A binding inhibition immunoassay was employed. Antibody concentration, assay buffer and regeneration solution have been optimized in order to reach the best performance and the lower non-specific binding of the matrix components to the sensor surface. The lowest detection limit was 6 ng/mL, with a working range covering the physiological range. Reproducibility of the assay was excellent with both intra-assay and inter-assay relative standard deviations <5%, while a variation of 2.19% was obtained employing different sensor chips. Reutilization of the sensor surface allows its continuous use over 50 measurements with a signal drop <20%. The SPR immunoassay results were validated using enzyme-linked immunosorbent assay (ELISA) showing an excellent correlation (R2 = 0.985). A portable and fully automated system (Sensia SL) was employed in this work. This is the first SPR biosensor assay capable of detecting relevant concentrations of a clinical analyte in serum. This study shows the potentials of this device as a diagnostic tool for the detection of multiple clinical analytes.
Description:6 páginas, 4 figuras, 1 tabla.
Publisher version (URL):http://dx.doi.org/10.1016/j.talanta.2009.01.012
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