Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/31375
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | MiRNA expression profile of human subcutaneous adipose and during adipocyte differentiation |
Autor: | Ortega, Francisco J.; Moreno-Navarrete, José Maria; Ferrer, Anna; Ricart, Wifredo; Peral, Belén CSIC ORCID; Fernández-Real, José M. | Palabras clave: | Microrna expression Tissue cellularity Cell-death Fat-cell Adipogenesis Obesity Number Tumorigenesis Genes Size |
Fecha de publicación: | 2-feb-2010 | Editor: | Public Library of Science | Citación: | PLoS ONE 5(2): e9022 (2010) | Resumen: | [Background]: Potential regulators of adipogenesis include microRNAs (miRNAs), small non-coding RNAs that have been recently shown related to adiposity and differentially expressed in fat depots. However, to date no study is available, to our knowledge, regarding miRNAs expression profile during human adipogenesis. Thereby, the aim of this study was to investigate whether miRNA pattern in human fat cells and subcutaneous adipose tissue is associated to obesity and co-morbidities and whether miRNA expression profile in adipocytes is linked to adipogenesis. [Methodology/Principal Findings]: We performed a global miRNA expression microarray of 723 human and 76 viral mature miRNAs in human adipocytes during differentiation and in subcutaneous fat samples from non-obese (n=6) and obese with (n=9) and without (n=13) Type-2 Diabetes Mellitus (DM-2) women. Changes in adipogenesis-related miRNAs were then validated by RT-PCR. Fifty of 799 miRNAs (6.2%) significantly differed between fat cells from lean and obese subjects. Seventy miRNAs (8.8%) were highly and significantly up or down-regulated in mature adipocytes as compared to pre-adipocytes. Otherwise, 17 of these 799 miRNAs (2.1%) were correlated with anthropometrical (BMI) and/or metabolic (fasting glucose and/or triglycerides) parameters. We identified 11 miRNAs (1.4%) significantly deregulated in subcutaneous fat from obese subjects with and without DM-2. Interestingly, most of these changes were associated with miRNAs also significantly deregulated during adipocyte differentiation. [Conclusions/Significance]: The remarkable inverse miRNA profile revealed for human pre-adipocytes and mature adipocytes hints at a closely crosstalk between miRNAs and adipogenesis. Such candidates may represent biomarkers and therapeutic targets for obesity and obesity-related complications. | Descripción: | This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0009022 | URI: | http://hdl.handle.net/10261/31375 | DOI: | 10.1371/journal.pone.0009022 | ISSN: | 1932-6203 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
MiRNA.pdf | 377,32 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
161
checked on 26-mar-2024
SCOPUSTM
Citations
319
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
302
checked on 27-feb-2024
Page view(s)
403
checked on 24-abr-2024
Download(s)
292
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.