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Título: | ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3 |
Autor: | Horiuchi, Horiuchi; Silverman, Neal; Piña, Benjamín CSIC ORCID ; Marcus, Gregory A.; Guarente, Leonard | Fecha de publicación: | jun-1997 | Editor: | American Society for Microbiology | Citación: | Molecular and Cellular Biology 17(6): 3220-3228 (1997) | Resumen: | The ADA genes encode factors which are proposed to function as transcriptional coactivators. Here we describe the cloning, sequencing, and initial characterization of a novel ADA gene, ADA1. Similar to the previously isolated ada mutants, ada1 mutants display decreases in transcription from various reporters. Furthermore, ADA1 interacts with the other ADAs in the ADA/GCN5 complex as demonstrated by partial purification of the complex and immunoprecipitation experiments. We estimate that the complex has a molecular mass of approximately 2 MDa. Previously, it had been demonstrated that ada5 mutants displayed more severe phenotypic defects than the other ada mutants (G. A. Marcus, J. Horiuchi, N. Silverman, and L. Guarente, Mol. Cell. Biol. 16:3197-3205, 1996; S. M. Roberts and F. Winston, Mol. Cell. Biol. 16:3206-3213, 1996). ada1 mutants display defects similar to those of ada5 mutants and different from those of the other mutants with respect to promoters affected, inositol auxotrophy, and Spt- phenotypes. Thus, the ADAs can be separated into two classes, suggesting that the ADA/GCN5 complex may have two separate functions. We present a speculative model on the possible roles of the ADA/GCN5 complex. | Descripción: | 9 pages, 5 figures, 3 tables.-- PMID: 9154821 [PubMed].-- PMCID: PMC232175. | Versión del editor: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC232175 | URI: | http://hdl.handle.net/10261/31237 | ISSN: | 0270-7306 | E-ISSN: | 1098-5549 |
Aparece en las colecciones: | (IDAEA) Artículos |
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