English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/3074
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Mitotic recombination and genetic changes in Saccharomyces cerevisiae during wine fermentation

AuthorsPuig, Sergi ; Querol, Amparo ; Barrio, Eladio ; Pérez Ortín, José E.
KeywordsMitotic recombination
wine fermentation
vinification
Saccharomyces cerevisiae
Issue DateMay-2000
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 66 (5) : 2057-2061 (2000)
AbstractNatural strains of Saccharomyces cerevisiae are prototrophic homothallic yeasts that sporulate poorly, are often heterozygous, and may be aneuploid. This genomic constitution may confer selective advantages in some environments. Different mechanisms of recombination, such as meiosis or mitotic rearrangement of chromosomes, have been proposed for wine strains. We studied the stability of the URA3 locus of a URA3/ura3 wine yeast in consecutive grape must fermentations. ura3/ura3 homozygotes were detected at a rate of 1 x 10(-5) to 3 x 10(-5) per generation, and mitotic rearrangements for chromosomes VIII and XII appeared after 30 mitotic divisions. We used the karyotype as a meiotic marker and determined that sporulation was not involved in this process. Thus, we propose a hypothesis for the genome changes in wine yeasts during vinification. This putative mechanism involves mitotic recombination between homologous sequences and does not necessarily imply meiosis.
URIhttp://hdl.handle.net/10261/3074
Appears in Collections:(IATA) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.