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Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei.
|Authors:||Nissen, Lorenzo; Yebra, María Jesús ; Sgobarti, Barbara; Pérez Martínez, Gaspar|
|Publisher:||Associazione Nazionale Biotecnologi Italiani|
|Citation:||Biofutura 2005, Bologna, 8-10 Aprile 2005|
|Abstract:||Sorbitol is a sugar polyol claimed to have health-promoting properties. D-sorbitol-6-phosphate dehydrogeanse (StolPDh) is required for sorbose and sorbitol metabolism in Lactobacillus casei. StolPDh catalyzes the oxidation of Sorbitol-6-phosphate and also the reverse reaction, or reather, the reduction of fructose-6-phosphate with NAD+ regeneration. In order to test this function in vivo, a new food grade recombinant strain of L. casei was constructed by the integration of a StolPDh-encoding gene (gutF) in the chromosomal lactose operon. Lactose induction of gutF expression was achieved and sorbitol production could be detected by 13C-nuclear magnetic resonance analys, upon addiction of glucose to resting cells. Additional inactivationn of L-lactate dehydrogenase led to higher sorbitol production. These outcomes indicates that the accumulation of the glycolytic precursor, fructose-6-phosphate and an high NAD+/NADH ratio were the limiting factors for sorbitol accumulation.|
|Appears in Collections:||(IATA) Comunicaciones congresos|