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dc.contributor.authorGonzález, Víctor M.-
dc.contributor.authorRodríguez Moreno, Luis-
dc.contributor.authorCenteno, Emilio-
dc.contributor.authorBenjak, Andrej-
dc.contributor.authorGarcía-Mas, Jordi-
dc.contributor.authorPuigdomènech, Pere-
dc.contributor.authorAranda, Miguel A.-
dc.date.accessioned2010-12-14T10:40:55Z-
dc.date.available2010-12-14T10:40:55Z-
dc.date.issued2010-11-05-
dc.identifier.citationBMC Genomics 11:618 (2010)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/29972-
dc.description11 pages, 1 figure, 6 tables.-- PMID: 21054843 [PubMed].-- Supporting information available at: http://www.biomedcentral.com/1471-2164/11/618/additionales_ES
dc.description.abstract[Background] Although melon (Cucumis melo L.) is an economically important fruit crop, no genome-wide sequence information is openly available at the current time. We therefore sequenced BAC-ends representing a total of 33,024 clones, half of them from a previously described melon BAC library generated with restriction endonucleases and the remainder from a new random-shear BAC library.es_ES
dc.description.abstract[Results] We generated a total of 47,140 high-quality BAC-end sequences (BES), 91.7% of which were paired-BES. Both libraries were assembled independently and then cross-assembled to obtain a final set of 33,372 nonredundant, high-quality sequences. These were grouped into 6,411 contigs (4.5 Mb) and 26,961 non-assembled BES (14.4 Mb), representing ~4.2% of the melon genome. The sequences were used to screen genomic databases, identifying 7,198 simple sequence repeats (corresponding to one microsatellite every 2.6 kb) and 2,484 additional repeats of which 95.9% represented transposable elements. The sequences were also used to screen expressed sequence tag (EST) databases, revealing 11,372 BES that were homologous to ESTs. This suggests that ~30% of the melon genome consists of coding DNA. We observed regions of microsynteny between melon paired-BES and six other dicotyledonous plant genomes.es_ES
dc.description.abstract[Conclusion] The analysis of nearly 50,000 BES from two complementary genomic libraries covered ~4.2% of the melon genome, providing insight into properties such as microsatellite and transposable element distribution, and the percentage of coding DNA. The observed synteny between melon paired-BES and six other plant genomes showed that useful comparative genomic data can be derived through large scale BAC-end sequencing by anchoring a small proportion of the melon genome to other sequenced genomes.es_ES
dc.description.sponsorshipThis project was carried out in the frame of the MELONOMICS project (2009-2012) of the Fundación Genoma España, and also supported by funding of the Consolider-Ingenio 2010 Programme of the Spanish Ministerio de Ciencia e Innovación (CSD2007-00036 "Center for Research in Agrigenomics").es_ES
dc.language.isoenges_ES
dc.publisherBioMed Centrales_ES
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccesses_ES
dc.titleGenome-wide BAC-end sequencing of Cucumis melo using two BAC librarieses_ES
dc.typeArtículoes_ES
dc.identifier.doi10.1186/1471-2164-11-618-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1186/1471-2164-11-618es_ES
dc.identifier.e-issn1471-2164-
dc.contributor.funderFundación Genoma España-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
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