Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/29028
Share/Impact:
Título : Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris
Autor : López García, Belén, Moreno, Ana Beatriz, San Segundo, Blanca, Ríos, Vivian de los, Manning, James M., Gavilanes, José G., Martínez-del-Pozo, Álvaro
Palabras clave : Antifungal
Antimicrobial
Cysteine-rich
Peptide
Fecha de publicación : Apr-2010
Editor: Elsevier
Resumen: The mould Aspergillus giganteus produces a basic, low molecular weight protein (AFP) showing in vitro and in vivo antifungal properties against important plant pathogens. AFP is secreted as an inactive precursor containing an amino-terminal extension of six amino acids (If-AFP) which is later removed to produce the active protein. The molecular basis to explain this behavior and the features that determine the fungal specificity of this protein are not completely solved. In this work, the mature AFP (AFP*) and a version of AFP with an extended amino-terminal (proAFP) have been cloned and produced in the yeast Pichia pastoris. The two proteins have been purified to homogeneity and characterized from structural and functional points of view. AFP* produced is practically indistinguishable from the natural fungal protein in terms of its spectroscopic and antifungal properties while proAFP is mostly inactive under identical assay conditions. The availability of an active AFP protein produced in P. pastoris will allow getting further insights into the mode of action and targeting specificity of AFP by using site-directed mutagenesis approaches.
Descripción : El pdf del artículo es la versión pre-print.
Versión del editor: http://dx.doi.org/10.1016/j.pep.2009.11.002
URI : http://hdl.handle.net/10261/29028
ISSN: 1046-5928
DOI: 10.1016/j.pep.2009.11.002
Citación : Protein Expression and Purification 70(2): 206-210 (2010)
Appears in Collections:(CRAG) Artículos
(CIB) Artículos

Files in This Item:
File Description SizeFormat 
Aspergillus giganteus.pdf245,67 kBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.