Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/28885
Share/Impact:
Título : Genomic characterization of putative allergen genes in peach/almond and their synteny with apple
Autor : Chen, Lin, Zhang, Shuiming, Illa, Eudald, Song, Lijuan, Wu, Shandong, Howad, Werner, Arús, Pere, Van de Weg, Eric, Chen, Kunsong, Gao, Zhongshan
Fecha de publicación : 17-Nov-2008
Editor: BioMed Central
Citación : BMC Genomics 9: 543 (2008)
Resumen: BACKGROUND: Fruits from several species of the Rosaceae family are reported to cause allergic reactions in certain populations. The allergens identified belong to mainly four protein families: pathogenesis related 10 proteins, thaumatin-like proteins, lipid transfer proteins and profilins. These families of putative allergen genes in apple (Mal d 1 to 4) have been mapped on linkage maps and subsequent genetic study on allelic diversity and hypoallergenic traits has been carried out recently. In peach (Prunus persica), these allergen gene families are denoted as Pru p 1 to 4 and for almond (Prunus dulcis)Pru du 1 to 4. Genetic analysis using current molecular tools may be helpful to establish the cause of allergenicity differences observed among different peach cultivars. This study was to characterize putative peach allergen genes for their genomic sequences and linkage map positions, and to compare them with previously characterized homologous genes in apple (Malus domestica).
RESULTS: Eight Pru p/du 1 genes were identified, four of which were new. All the Pru p/du 1 genes were mapped in a single bin on the top of linkage group 1 (G1). Five Pru p/du 2 genes were mapped on four different linkage groups, two very similar Pru p/du 2.01 genes (A and B) were on G3, Pru p/du 2.02 on G7,Pru p/du 2.03 on G8 and Pru p/du 2.04 on G1. There were differences in the intron and exon structure in these Pru p/du 2 genes and in their amino acid composition. Three Pru p/du 3 genes (3.01-3.03) containing an intron and a mini exon of 10 nt were mapped in a cluster on G6. Two Pru p/du 4 genes (Pru p/du 4.01 and 4.02) were located on G1 and G7, respectively. The Pru p/du 1 cluster on G1 aligned to the Mal d 1 clusters on LG16; Pru p/du 2.01A and B on G3 to Mal d 2.01A and B on LG9; the Pru p/du 3 cluster on G6 to Mal d 3.01 on LG12; Pru p/du 4.01 on G1 to Mal d 4.03 on LG2; and Pru p/du 4.02 on G7 to Mal d 4.02 on LG2.
CONCLUSION: A total of 18 putative peach/almond allergen genes have been mapped on five linkage groups. Their positions confirm the high macro-synteny between peach/almond and apple. The insight gained will help to identify key genes causing differences in allergenicity among different cultivars of peach and other Prunus species.
Descripción : 15 pages, 6 figures, 4 tables.-- PMID: 19014629 [PubMed].-- PMCID: PMC2621206.
Versión del editor: http://dx.doi.org/10.1186/1471-2164-9-543
URI : http://hdl.handle.net/10261/28885
DOI: 10.1186/1471-2164-9-543
Citación : BMC Genomics 9: 543 (2008)
Appears in Collections:(IQAC) Artículos

Files in This Item:
File Description SizeFormat 
Chen_Lin_et_al.pdf476,21 kBAdobe PDFView/Open
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.