DSpace

Digital.CSIC > Ciencia y Tecnologías Químicas > Instituto de Química Avanzada de Cataluña (IQAC) > (IQAC) Artículos >

Share

EndNote

Links

Closed Access item Molecular cloning and pattern of expression of an alpha-L-fucosidase gene from pea seedlings

Authors:Augur, Christopher
Stiefel, Virginia
Darvin, Alan
Albersheim, Peter
Puigdomènech, Pere
Issue Date:Oct-1995
Publisher:American Society for Biochemistry and Molecular Biology
Citation:Journal of Biological Chemistry 270(42): 24839-24843 (1995)
Abstract:Alpha-L-Fucosidase is a cell wall protein purified from pea (Pisum sativum) epicotyls. The alpha-L-fucosidase hydrolyzes terminal fucosyl residues from oligosaccharides of plant cell wall xyloglucan. alpha-L-Fucosidase may be an important factor in plant growth regulation, as it inactivates fucose-containing xyloglucan oligosaccharides that inhibit growth of pea stem segments. The amino acid sequences of the NH2-terminal region and one internal peptide were used to design redundant oligonucleotides that were utilized as primers in a polymerase chain reaction (PCR) with cDNA, generated from pea mRNA, as the template. A specific PCR amplification product containing 357 base pairs was isolated, cloned, and sequenced. The deduced amino acid sequence included the two peptides used to design the primers for PCR plus two other peptides obtained by proteinase digestion of alpha-L-fucosidase. No sequence homology to other alpha-L-fucosidases was apparent, although the NH2-terminal region is strongly homologous to Kunitz-type trypsin inhibitors. cDNA and genomic copies were isolated and sequenced. In pea, the gene is present in two or three copies. Its mRNA is present in roots, leaves, and elongating shoots. The spatial pattern of expression of the alpha-L-fucosidase was determined by in situ hybridization.
Description:5 pages, 6 figures, 1 table.-- PMID: 7559605 [PubMed].
Publisher version (URL):http://www.jbc.org/content/270/42/24839
URI:http://hdl.handle.net/10261/28574
ISSN:0021-9258
E-ISSNmetadata.dc.identifier.doi = DOI:1083-351X
Appears in Collections:(IQAC) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.