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Title: | Histochemical and immunocytochemical study of gonadotropic pituitary cells of the killifish, Fundulus heteroclitus during annual reproductive cycle |
Other Titles: | Estudio histoquímico e inmunohistoquímico de las células gonadotropas de la pituitaria de Fundulus heteroclitus | Authors: | Sarasquete, Carmen CSIC ORCID ; Muñoz-Cueto, José Antonio; González de Canales, María Luisa; García-García, A. CSIC ORCID CVN; Rodríguez-Gómez, F. J.; Piñuela, C.; Rendón, C.; Rodríguez Martínez, Ramón B. | Keywords: | Histochemistry Immunocytochemistry Pituitary Gonadotropic cells Somatotropic cells GSI Fundulus heteroclitus Histoquímica Inmuno citoquímica Pituitaria Células gonadotropas Células somatotropas IGS |
Issue Date: | 1997 | Publisher: | Consejo Superior de Investigaciones Científicas (España) | Citation: | Scientia Marina 61(4): 439-449 (1997) | Abstract: | [EN] A selection of various conventional histochemical techniques, lectins and antibodies were used to identify the gonadotropic and somatotropic cells in the killifish, Fundulus heteroclitus pituitary. Distribution and variation of the gonadotropic cells and some histochemical reactions (PAS and Alcian Blue) largely parallel the cyclic changes in the gonads (GSI). Pituitary cells located in the ventral proximal pars distalis (PPD) and pars intermedia (PI) of the pituitary of the killifish, contain basophilic granules, which were positive to Alcian Blue and PAS reactions. These cells showed reactivity to different lectins, indicating the presence of Man (mannose) and/or Glc (glucose); GlcNAc (N-acetylglucosamine) and NANA (sialic acids); Gal (galactose) as well as GalNAc (N-acetylgalactosamine) sugar residues of glycoconjugates. These pituitary basophilic cells were stained with anti carp a,b GTH, and specially with anti carp b GTH; the maximal and minimal immunostaining, against the first antisera used, were observed during maturation/spawning period and during postspawning/resting phase, respectively. On the other hand, acidophilic cells located in the dorsal proximal pars distalis (PPD) of the pituitary gland of the killifish, were selectively stained with eosin, light green and/or orange G, when Haematoxylin-eosin, Haematoxylin-V.O.F and/or Alcian Blue-PAS-Orange G techniques were performed, respectively. These acidophilic cells were negative to PAS, Alcian Blue and lectins reactions; they contain proteins rich in different aminoacids and showed specific immunostaining against anti recombinant seabream growth hormone (anti rsbGH). [ES] Un grupo de diferentes técnicas histoquímicas convencionales, lectinas y anticuerpos han sido utilizadas para identificar las células gonadotropas y somatotropas de la pituitaria del fundulus, Fundulus heteroclitus. La distribución y variación de las células gonadotropas y algunas reacciones histoquímicas (PAS y Azul Alcián) se producen de forma paralela a los cambios cíclicos anuales que experimentan las gonadas (GSI). Las células localizadas en la porción ventral de la proximal pars distalis (PPD) y en la pars intermedia (PI) de la pituitaria de Fundulus heteroclitus contienen gránulos basófilos y positivos a las reacciones de Azul Alcián y PAS. Estas células fueron reactivas con diferentes lectinas, indicando la presencia de glicoconjugados con residuos de Man (manosa) y/o Glc (glucosa); GlcNAc (N-acetilglucosamina) and NANA (ácidos siálicos); Gal (galactosa), así como GalNAc (N-acetilgalactosamina). Estas células basófilas, positivas con el anticuerpo anti carpa α,β GTH y especialmente con anti carp β GTH, mostraron las máximas y mínimas inmunotinciones, con el primer anticuerpo usado, durante el periodo de maduración/puesta y durante el periodo de postpuesta-reposo, respectivamente. Por otra parte, las células acidófilas localizadas en la porción dorsal de la proximal pars distalis (PPD) de la pituitaria de Fundulus heteroclitus, fueron selectivamente teñidas con eosina/ verde-luz y/u orange G (Hematoxilina-eosina/ Hematoxilina-V.O.F. y/o Azul Alcián-PAS-Orange G). Estas células acidófilas que contienen proteinas ricas en diferentes aminoácidos, fueron negativas a las reacciones del PAS, Azul Alcián y lectinas y mostraron inmunotinción específica frente al antisuero, anti hormona de crecimiento recombinante de dorada (anti rsbGH). |
Description: | 11 páginas, 4 figuras, 2 tablas. | Publisher version (URL): | http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/2874/ | URI: | http://hdl.handle.net/10261/28220 | ISSN: | 0214-8358 | E-ISSN: | 1886-8134 |
Appears in Collections: | (ICMAN) Artículos (CIB) Artículos |
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