Please use this identifier to cite or link to this item:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

High-Q dynamic force microscopy in liquid and its application to living cells

AuthorsTamayo de Miguel, Francisco Javier CSIC ORCID; Humphris, A. D. L.; Owen, R. J.; Miles, M. J.
Issue DateJul-2001
PublisherBiophysical Society
CitationBiophys J. 2001 July; 81(1): 526–537.
AbstractWe present a new dynamic force microscopy technique for imaging in liquids in the piconewton regime. The low quality factor (Q) of the cantilever is increased up to three orders of magnitude by the implementation of a positive feedback control. The technique also includes a phase-locked loop unit to track the resonance of the cantilever. Experiments and computer simulations indicate that the tip-sample forces are below 100 pN, about two orders of magnitude lower than in conventional tapping mode atomic force microscopy. Furthermore, the spectroscopic ability is greatly enhanced. Either the phase shift or the resonant frequency shows a high sensitivity to variations in either the energy dissipation or conservative interactions between the tip and the sample, respectively. The potential of this technique is demonstrated by imaging living cells.
Publisher version (URL)
Appears in Collections:(IMN-CNM) Artículos

Files in This Item:
File Description SizeFormat
tamayo.pdf1,26 MBAdobe PDFThumbnail
Show full item record
Review this work

Page view(s)

checked on Jul 4, 2022


checked on Jul 4, 2022

Google ScholarTM


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.