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High-Q dynamic force microscopy in liquid and its application to living cells

AuthorsTamayo de Miguel, Francisco Javier ; Humphris, A. D. L.; Owen, R. J.; Miles, M. J.
Issue DateJul-2001
PublisherBiophysical Society
CitationBiophys J. 2001 July; 81(1): 526–537.
AbstractWe present a new dynamic force microscopy technique for imaging in liquids in the piconewton regime. The low quality factor (Q) of the cantilever is increased up to three orders of magnitude by the implementation of a positive feedback control. The technique also includes a phase-locked loop unit to track the resonance of the cantilever. Experiments and computer simulations indicate that the tip-sample forces are below 100 pN, about two orders of magnitude lower than in conventional tapping mode atomic force microscopy. Furthermore, the spectroscopic ability is greatly enhanced. Either the phase shift or the resonant frequency shows a high sensitivity to variations in either the energy dissipation or conservative interactions between the tip and the sample, respectively. The potential of this technique is demonstrated by imaging living cells.
Publisher version (URL)http://www.biophysj.org/cgi/content/full/81/1/526
Appears in Collections:(IMN-CNM) Artículos
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