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Open Access item Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing

Authors:Sánchez del Río, José
Carrascosa, Laura G.
Blanco, Francisco
Moreno, M.
Berganzo Ruiz, Javier
Calle Martín, Ana
Domínguez, Carlos
Lechuga, Laura M.
Keywords:Biosensor, Mach-Zehnder interferometer, Silicon microtechnology, DNA biosensing, Microfluidics, lab-on-a-chip microsystem
Issue Date:14-Feb-2007
Publisher:Society of Photo-Optical Instrumentation Engineers
Citation:Proceedings of SPIE 6477: 64771B (2007)
Abstract:In order to solve the drawbacks of sensitivity and portability in optical biosensors we have developed ultrasensitive and miniaturized photonic silicon sensors able to be integrated in a "lab-on-a-chip" microsystem platform. The sensors are integrated Mach-Zehnder interferometers based on TIR optical waveguides (Si/SiO2/Si3N4) of micro/nanodimensions. We have applied this biosensor for DNA testing and for detection of single nucleotide polymorphisms at BRCA-1 gene, involved in breast cancer development, without target labeling. The oligonucleotide probe is immobilized by covalent attachment to the sensor surface through silanization procedures. The hybridization was performed for different DNA target concentrations showing a lowest detection limit at 10 pM. Additionally, we have detected the hybridization of different concentrations of DNA target with two mismatching bases corresponding to a mutation of the BRCA-1 gene. Following the way of the lab-on-a-chip microsystem, integration with the microfluidics has been achieved by using a novel fabrication method of 3-D embedded microchannels using the polymer SU-8 as structural material. The optofluidic chip shows good performances for biosensing.
Description:9 páginas, 7 figuras, 1 tabla.-- Trabajo presentado en la conferencia "Silicon Photonics II", 22-Enero-2007, San Jose, CA, USA; Editores: Joel A. Kubby, Graham T. Reed.
Publisher version (URL):http://dx.doi.org/10.1117/12.713977
Appears in Collections:(IMM-CNM) Artículos

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