Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/27666
Share/Export:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

DNA-sequence and metal-ion specificity of the formation of *H-DNA

AuthorsBernués, Jordi CSIC ORCID ; Beltrán, Ramón; Casasnovas, José María ; Azorín, Ferran CSIC ORCID
Issue Date25-Jul-1990
PublisherOxford University Press
CitationNucleic Acids Research 18(14): 4067-4073 (1990)
AbstractThe homopyrimidine-homopurine sequence d(CT/GA)22 undergoes, in the presence of zinc ions, transition to an altered DNA conformation (*H-DNA) which is neither H-DNA nor B-DNA. *H-DNA is characterized by a peculiar chemical reactivity pattern in which most of the polypyrimidine strand is hyperreactive to osmium tetroxide and the central part of the polypurine strand is sensitive to diethylpyrocarbonate. Formation of *H-DNA is specific of metal-ion. *H-DNA is detected in the presence of Zn++, Cd++ and Mn++. The efficiency on promoting the transition is in the order of Zn++ greater than Cd++ much greater than Mn++. Formation of *H-DNA is also specific of nucleotide sequence. From all the different homopolymeric sequences tested only the d(CT/GA)22 sequence showed the zinc-induced transition to *H-DNA. These results suggest that stabilization of *H-DNA involves the formation of a specific complex between the metal-ion and the nucleotide sequence. The biological relevance of these results is discussed in view of the important role that zinc ions play on many nucleic acids processes.
Description7 pages, 4 figures.-- PMID: 2377452 [PubMed].-- PMCID: PMC331161.
Publisher version (URL)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC331161
URIhttp://hdl.handle.net/10261/27666
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(IDAEA) Artículos




Files in This Item:
File Description SizeFormat
Bernues_Jordi_et_al.pdf2,57 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

Page view(s)

325
checked on May 24, 2022

Download(s)

176
checked on May 24, 2022

Google ScholarTM

Check


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.