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dc.contributor.authorVignols, Florence-
dc.contributor.authorRigau, Joan-
dc.contributor.authorTorres, M. A.-
dc.contributor.authorCapellades, Montserrat-
dc.contributor.authorPuigdomènech, Pere-
dc.identifier.citationPlant Cell 7(4): 407-416 (1996)en_US
dc.description10 pages, 5 figures.-- PMID: 7773015 [PubMed].-- PMCID: PMC160792.en_US
dc.description.abstractThe brown midrib mutations are among the earliest described in maize. Plants containing a brown midrib mutation exhibit a reddish brown pigmentation of the leaf midrib starting when there are four to six leaves. These mutations are known to alter lignin composition and digestibility of plants and therefore constitute prime candidates in the breeding of silage maize. Here, we show that two independent brown midrib3 (bm3) mutations have resulted from structural changes in the COMT gene, which encodes the enzyme O-methyltransferase (COMT; EC, involved in lignin biosynthesis. Our results indicate that the bm3-1 allele (the reference mutant allele) has arisen from an insertional event producing a COMT mRNA altered in both size and amount. By sequencing a COMT cDNA clone obtained from bm3-1 maize, a retrotransposon with homology to the B5 element has been found to be inserted near the junction of the 3' coding region of the COMT gene intron. The second bm3 allele, bm3-2, has resulted from a deletion of part of the COMT gene. These alterations of the COMT gene were confirmed by DNA gel blot and polymerase chain reaction amplification analyses. These results clearly demonstrate that mutations at the COMT gene give a brown midrib3 phenotype. Thus, the gene genetically recognized as bm3 is the same as the one coding for COMT.en_US
dc.description.sponsorshipThis work was supported by Grant No. Bio94/734 from Plan Nacional de Investigación Científica y Técnica. FV. is a postdoctoral fellow of the European Community Human Capital and Mobility Program. M.C. was the recipient of a fellowship from the Departament d'Ensenyament of the Generalitat de Catalunya.en_US
dc.format.extent2825034 bytes-
dc.publisherAmerican Society of Plant Physiologistsen_US
dc.titleThe brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferaseen_US
dc.description.peerreviewedPeer revieweden_US
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