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The obtention of simian virus 40 recombinants carrying d(CG.GC)n, d(CA.GT)n and d(CT.GA)n sequences. Stability of the inserted simple repeating sequences

AuthorsCasasnovas, José María; Ellison, Michael J.; Rodríguez-Campos, Antonio; Azorín, Ferran
Issue DateSep-1987
PublisherBlackwell Publishing
CitationEuropean Journal of Biochemistry 167(3): 489-492 (1987)
AbstractA general strategy for the introduction of simple repeating DNA sequences into the simian virus 40 (SV40) has been developed. SV40 recombinants carrying d(CG · GC)5, d(CA · GT)30 or d(CT · GA)22 insertions at either the TaqI site (position 4739) or the HpaII site (position 346) were obtained and the stability of the inserted DNA sequences studied. The palindromic potentially Z-DNA-forming d(CG · GC)n sequence was found to be highly unstable when compared to either d(CA · GT)n or d(CT · GA)n.
Description4 pages, 3 figures.-- PMID: 2820728 [PubMed].-- Available online Mar 3, 2005.
Publisher version (URL)http://dx.doi.org/10.1111/j.1432-1033.1987.tb13363.x
Appears in Collections:(IDAEA) Artículos
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