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Closed Access item Molecular variability and phylogeny of Schistonchus caprifici (Gasperrini, 1864) Cobb, 1927 (Nematoda: Aphelenchoididae) from Italy and Spain
|Authors:||Luca, F. de|
Palomares Rius, Juan E.
|Citation:||Nematology 12(4) :641-648 (2010)|
|Abstract:||Molecular characterisation of four Schistonchus caprifici
populations (two from Spain and two from Italy), using the partial
18S, the D2-D3 expansion segments of 28S of ribosomal DNA
(rDNA) and the partial mitochondrial gene for cytochrome c
oxidase I (mtCOI), is provided. Amplicons from partial mtCOI
were analysed using 'Single Strand Conformation Polymorphism'
(SSCP) analysis in order to rapidly screen for genetic (haplotypic)
variability. SSCP analysis of mtCOI revealed a close relationship
between the Spanish and Italian populations with low intrapopulation
variability and only two haplotypes were detected.
Mitochondrial COI and ribosomal genes were analysed using
Maximum Likelihood and Bayesian inference to reconstruct
phylogenetic relationships within S. caprifici and other members of
the family Aphelenchoididae. The phylogenetic analysis based on
mtCOI showed no geographic variability among Italian, Spanish
and Turkish populations. The results of phylogenetic analyses
based on the D2-D3 expansion segments of 28S rDNA region and
the partial 18S rDNA genes revealed a closer phylogenetic
relationship between S. aureus, S. laevigatus, S. virens and S.
centerae, whilst S. caprifici and S. guangzhouensis clustered
separately. The phylogeny of the genus Schistonchus was well related to some morphological characters, such as position of
excretory pore, presence/absence of labial disc and number and position of caudal papillae. The monophyly of the genus
Schistonchus was rejected by the Shimodaira-Hasegawa test
based on tree topologies.|
|E-ISSNmetadata.dc.identifier.doi = DOI:||1568-5411|
|Appears in Collections:||(IAS) Artículos|
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