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dc.contributor.authorSánchez, Olga-
dc.contributor.authorGasol, Josep M.-
dc.contributor.authorMassana, Ramon-
dc.date.accessioned2010-08-02T09:20:14Z-
dc.date.available2010-08-02T09:20:14Z-
dc.date.issued2007-09-
dc.identifier.citationApplied and Environmental Microbiology 73(18): 5962-5967 (2007)en_US
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/26731-
dc.description5 pages, 3 figures, 2 tablesen_US
dc.description.abstractAn annual seasonal cycle of composition of a bacterioplankton community in an oligotrophic coastal system was studied by denaturing gradient gel electrophoresis (DGGE) using five different primer sets. Analysis of DGGE fingerprints showed that primer set 357fGC-907rM grouped samples according to seasons. Additionally, we used the set of 16S rRNA genes archived in the RDPII database to check the percentage of perfect matches of each primer for the most abundant bacterial groups inhabiting coastal plankton communities. Overall, primer set 357fGC-907rM was the most suitable for the routine use of PCR-DGGE analyses in this environmenten_US
dc.description.sponsorshipThis work was supported by the Spanish projects MicroDiff (REN2001-2110/MAR), ESTRAMAR (CTM2004-12631/MAR), GENMuMAR (CTM2004-02586/MAR), TRAGUA (CSD2006-00044), TEC2006-13109-C03-02/MIC, MODIVUS (CTM2005-04975/MAR), and NoEs MABEF and Marine Genomics Europeen_US
dc.format.extent5867 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsclosedAccessen_US
dc.titleComparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communitiesen_US
dc.typeartículoen_US
dc.identifier.doi10.1128/AEM.00817-07-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1128/AEM.00817-07en_US
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