English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/26731
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities

AuthorsSánchez, Olga ; Gasol, Josep M. ; Massana, Ramon
Issue DateSep-2007
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 73(18): 5962-5967 (2007)
AbstractAn annual seasonal cycle of composition of a bacterioplankton community in an oligotrophic coastal system was studied by denaturing gradient gel electrophoresis (DGGE) using five different primer sets. Analysis of DGGE fingerprints showed that primer set 357fGC-907rM grouped samples according to seasons. Additionally, we used the set of 16S rRNA genes archived in the RDPII database to check the percentage of perfect matches of each primer for the most abundant bacterial groups inhabiting coastal plankton communities. Overall, primer set 357fGC-907rM was the most suitable for the routine use of PCR-DGGE analyses in this environment
Description5 pages, 3 figures, 2 tables
Publisher version (URL)http://dx.doi.org/10.1128/AEM.00817-07
URIhttp://hdl.handle.net/10261/26731
DOI10.1128/AEM.00817-07
ISSN0099-2240
Appears in Collections:(ICM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.