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Title

Potent Neurotoxic Action of the Shellfish Biotoxin Yessotoxin on Cultured Cerebellar Neurons

AuthorsPérez-Gómez, Anabel; Ferrero-Gutiérrez, Amaia; Novelli, Antonello; Franco, José M. CSIC; Paz, Beatriz CSIC; Fernández-Sánchez, M. Teresa
KeywordsYessotoxin
Neurotoxicity
Rat cerebellar neurons
Apoptosis
F-actin
Ion channels
Issue DateDec-2006
PublisherOxford University Press
CitationToxicological Sciences 90(1): 168-177 (2006)
AbstractYessotoxin (YTX) and its analogues are disulphated polyether compounds of increasing occurrence in seafood. The biological effects of these algal toxins on mammals and the risk associated to their ingestion have not been clearly established. We have used primary cultures of rat cerebellar neurons to investigate whether YTX affected survival and functioning of central nervous system neurons. Exposure to YTX (25 nM) caused first (8 h) weakening, granulation, and fragmentation of neuronal network, and later (48 h) complete disintegration of neurites and extensive neuronal death, with a significant decrease in the amount of filamentous actin. The concentration of YTX that reduced by 50% the maximum neuronal survival (EC5048) was 20 nM. Lower toxin concentrations (15 nM) also caused visible signs of toxicity affecting neuronal network primarily. Removal of YTX after 5 h exposure delayed the onset of neurotoxicity but did not prevent neuronal degeneration and death. YTX induced a two-fold increase in cytosolic calcium that was prevented by the voltage-sensitive calcium channel antagonists nifedipine and verapamil. These antagonists were, however, completely ineffective in reducing neurotoxicity. Voltage-sensitive sodium channel antagonists saxitoxin and nefopam, and the NMDA receptor antagonist MK-801 also failed to prevent YTX neurotoxicity. Neuronal death by YTX involved typical hallmarks of apoptosis and required the synthesis of new proteins. Our data suggest neuronal tissue to be a vulnerable biological target for YTX. The potent neurotoxicity of YTX we report raises reasonable concern about the potential risk that exposure to YTX may represent for neuronal survival in vivo.
Description10 pages, 6 figures, 1 table.-- The PDF of this article is free.
Publisher version (URL)http://dx.doi.org/10.1093/toxsci/kfj064
URIhttp://hdl.handle.net/10261/26273
DOI10.1093/toxsci/kfj064
ISSN1096-6080
Appears in Collections:(IIM) Artículos




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