Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/26266
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production

AuthorsTafalla, Carolina; Figueras Huerta, Antonio ; Novoa, Beatriz CSIC ORCID
KeywordsNitric oxide
Lipopolysacharide
Macrophage
Viral hemorrhagic virus
Turbot Scophthalmus maximus
Macrophage activating factor
Tumor necrosis factor α
Issue DateOct-2001
PublisherInter Research
CitationDiseases of Aquatic Organisms 47(2): 101-107 (2001)
AbstractThe effect of viral hemorrhagic septicemia virus (VHSV) in vitro infection on the nitric oxide (NO) production by turbot Scophthalmus maximus kidney macrophages has been addressed in the past. Previously, we had determined that only a small fraction of turbot possess head kidney macrophages that respond to a single exposure of lipopolysaccharide (LPS) with NO production (LPS-responsive macrophages), whereas macrophage cultures from other individuals were not activated by LPS alone and needed a combination of stimuli to respond (LPS-non-responsive macrophages). In the current work, we examined the effect of VHSV on NO production by macrophages characterized as LPS-responsive macrophages or LPS-non-responsive macrophages. Combinations of LPS and tumor necrosis factor α (TNF-α) and macrophage-activating factor (MAF) were also used to stimulate the cells for NO production. The effect of VHSV on NO production depends on the response to LPS alone. When a low multiplicity of infection was used (1.78 x 10-3), the NO production in response to LPS in LPS-responsive macrophages was significantly decreased. However, LPS-non-responsive macrophage cultures produced NO when a combination of LPS and VHSV was used. In the case of a higher VHSV multiplicity of infection (1.78), no significant change was observed in LPS-non-responsive animals. Combinations of LPS with TNF- α, LPS with MAF, and TNF-α with MAF were used to induce NO production in LPS-non-responsive macrophages. In all these cases, VHSV suppressed NO production, although at a significant level only when a combination of TNF-α and MAF was used for the induction of NO.
Description7 pages, 4 figures.
Publisher version (URL)http://dx.doi.org/10.3354/dao047101
URIhttp://hdl.handle.net/10261/26266
DOI10.3354/dao047101
ISSN0177-5103
Appears in Collections:(IIM) Artículos




Files in This Item:
File Description SizeFormat
d047p101.pdf147,42 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

SCOPUSTM   
Citations

26
checked on May 23, 2022

WEB OF SCIENCETM
Citations

26
checked on May 20, 2022

Page view(s)

301
checked on May 23, 2022

Download(s)

333
checked on May 23, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.